The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Collection of Samples
2.2. Experimental Design
2.3. Statistical Analysis
3. Results
3.1. Comparison of the Post-Thaw Sperm Motility Parameters among the Different Activation Media
3.2. Differences between OF 100% and OF 50%: The Effect of Males, Females and Male–Female Interaction
3.3. Correlation among the Post-Thaw Sperm Motility Parameters Activated in Ovarian Fluid and Control Activation Solution
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatments | |||
---|---|---|---|
Sperm Traits | D-532 | OF 50% | OF 100% |
MOT (%) | 43.04 ± 13.59 b | 57.49 ± 14.00 a | 55.56 ± 13.83 ab |
VCL (µm/s) | 138.01 ± 18.74 a | 122.29 ± 32.01 a | 107.72± 27.69 b |
LIN (%) | 64.23 ± 7.79 a | 50.00 ± 8.55 b | 49.98 ± 9.90 b |
ALH (µm) | 2.49 ± 0.57 b | 3.21 ± 0.80 a | 2.84 ± 0.70 b |
BCF (Hz) | 4.28 ± 0.72 | 3.71 ± 0.84 | 3.72 ± 0.94 |
DSM (s) | 32.86 ± 6.33 b | 46.39 ± 9.60 a | 45.98 ± 10.84 a |
Random Effects | MOT | VCL | ALH | |||
---|---|---|---|---|---|---|
p | AIC | p | AIC | p | AIC | |
Male | *** | −154.41 | *** | 1308.1 | ** | 320.14 |
Female | * | −129.15 | 1346.2 | ** | 320.5 | |
Male–Female | *** | −216.94 | *** | 1329.2 | *** | 309.83 |
Fixed effect | ||||||
Treatment (OF 100% vs. OF 50%) | * | *** | *** |
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Rusco, G.; Di Iorio, M.; Esposito, S.; Antenucci, E.; Roncarati, A.; Iaffaldano, N. The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance. Vet. Sci. 2023, 10, 219. https://doi.org/10.3390/vetsci10030219
Rusco G, Di Iorio M, Esposito S, Antenucci E, Roncarati A, Iaffaldano N. The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance. Veterinary Sciences. 2023; 10(3):219. https://doi.org/10.3390/vetsci10030219
Chicago/Turabian StyleRusco, Giusy, Michele Di Iorio, Stefano Esposito, Emanuele Antenucci, Alessandra Roncarati, and Nicolaia Iaffaldano. 2023. "The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance" Veterinary Sciences 10, no. 3: 219. https://doi.org/10.3390/vetsci10030219