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Isolation, Characterization, and Agent-Based Modeling of Mesenchymal Stem Cells in a Bio-construct for Myocardial Regeneration Scaffold Design

1
College of Engineering, Universidad Autónoma de Occidente, Calle 25 115-85, Cali 760030, Colombia
2
School of Business and Engineering Vaud (HEIG-VD), University of Applied Sciences Western of Switzerland (HES-SO), Route de Cheseaux 1, CH-1400 Yverdon-les-Bains, Switzerland
*
Authors to whom correspondence should be addressed.
This paper is an extended version of our paper published in 2018 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), Madrid, Spain, 3–6 December 2018, doi:10.1109/BIBM.2018.8621314.
Received: 28 March 2019 / Revised: 16 May 2019 / Accepted: 16 May 2019 / Published: 19 May 2019
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Abstract

Regenerative medicine involves methods to control and modify normal tissue repair processes. Polymer and cell constructs are under research to create tissue that replaces the affected area in cardiac tissue after myocardial infarction (MI). The aim of the present study is to evaluate the behavior of differentiated and undifferentiated mesenchymal stem cells (MSCs) in vitro and in silico and to compare the results that both offer when it comes to the design process of biodevices for the treatment of infarcted myocardium in biomodels. To assess in vitro behavior, MSCs are isolated from rat bone marrow and seeded undifferentiated and differentiated in multiple scaffolds of a gelled biomaterial. Subsequently, cell behavior is evaluated by trypan blue and fluorescence microscopy, which showed that the cells presented high viability and low cell migration in the biomaterial. An agent-based model intended to reproduce as closely as possible the behavior of individual MSCs by simulating cellular-level processes was developed, where the in vitro results are used to identify parameters in the agent-based model that is developed, and which simulates cellular-level processes: Apoptosis, differentiation, proliferation, and migration. Thanks to the results obtained, suggestions for good results in the design and fabrication of the proposed scaffolds and how an agent-based model can be helpful for testing hypothesis are presented in the discussion. It is concluded that assessment of cell behavior through the observation of viability, proliferation, migration, inflammation reduction, and spatial composition in vitro and in silico, represents an appropriate strategy for scaffold engineering. View Full-Text
Keywords: agent-based modeling; biological system modeling; cell migration; cell viability; computational modeling; myocardium; myocardial infarction; scaffold; stem cells; tissue engineering agent-based modeling; biological system modeling; cell migration; cell viability; computational modeling; myocardium; myocardial infarction; scaffold; stem cells; tissue engineering
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Ramírez López, D.V.; Melo Escobar, M.I.; Peña-Reyes, C.A.; Rojas Arciniegas, Á.J.; Neuta Arciniegas, P.A. Isolation, Characterization, and Agent-Based Modeling of Mesenchymal Stem Cells in a Bio-construct for Myocardial Regeneration Scaffold Design. Data 2019, 4, 71.

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