Next Article in Journal
Thermal and Non-Thermal Physical Methods for Improving Polyphenol Extraction in Red Winemaking
Previous Article in Journal
Beverage Sensory Modification
Open AccessCommunication

Detection and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes Isolates from Milk Vending Machines in Croatia

1
Department of Hygiene, Technology and Food Safety, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
2
Food Institute, Faculty of Veterinary Medicine in Skopje, University “Ss’ Cyril and Methodius”, 1000 Skopje, Macedonia
3
Center for Food Safety, Croatian Agency for Agriculture and Food, Ivana Gundulića 36b, 31000 Osijek, Croatia
4
Department of Dairy Science, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Beverages 2019, 5(3), 46; https://doi.org/10.3390/beverages5030046
Received: 14 April 2019 / Revised: 9 July 2019 / Accepted: 16 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Milk)
The aim of the present study was to evaluate the persistence of Listeria monocytogenes in raw milk from vending machines, based on culture and molecular detection of pathogen and Pulsed-Field Gel Electrophoresis typing. From December 2015 to January 2017, a total of 319 milk samples from 36 vending machines were examined for the presence and count of L. monocytogenes by reference methods ISO 11290:1 and ISO 11290:2. Molecular detection of pathogens was performed by loop-mediated isothermal DNA amplification (LAMP) coupled with bioluminescence (Molecular Detection Assay). L. monocytogenes was detected by MDA in 14 milk samples (4.38%) from four farms, compared to eight positive samples (2.5%) retrieved by a reference ISO method. Cultivable L. monocytogenes isolates were subjected to Pulsed-Field Gel Electrophoresis typing and pulsotypes were compared with those obtained during the previous survey in Croatia (2014‒2015). It was found that identical PFGE patterns of L. monocytogenes occur in milk samples of the same producer over a three-year period, indicating the persistence of pathogens in raw milk vending machines. The results obtained support the need for more effective control of milk in the entire food chain. View Full-Text
Keywords: raw milk; milk vending machine; Listeria monocytogenes; Molecular Detection Assay; Pulsed-Field Gel Electrophoresis raw milk; milk vending machine; Listeria monocytogenes; Molecular Detection Assay; Pulsed-Field Gel Electrophoresis
Show Figures

Figure 1

MDPI and ACS Style

Zdolec, N.; Jankuloski, D.; Kiš, M.; Hengl, B.; Mikulec, N. Detection and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes Isolates from Milk Vending Machines in Croatia. Beverages 2019, 5, 46.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop