Next Article in Journal
Epidermal Electrodes with Ferrimagnetic/Conductive Properties for Biopotential Recordings
Next Article in Special Issue
Review of the Developments of Bacterial Medium-Chain-Length Polyhydroxyalkanoates (mcl-PHAs)
Previous Article in Journal
Decellularised Cartilage ECM Culture Coatings Drive Rapid and Robust Chondrogenic Differentiation of Human Periosteal Cells
Previous Article in Special Issue
PHB Producing Cyanobacteria Found in the Neighborhood—Their Isolation, Purification and Performance Testing
 
 
Article

Lab-Scale Cultivation of Cupriavidus necator on Explosive Gas Mixtures: Carbon Dioxide Fixation into Polyhydroxybutyrate

by 1,2 and 1,2,*
1
Austrian Centre of Industrial Biotechnology (ACIB), Krenngasse 37, A-8010 Graz, Austria
2
Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWI Graz, Petersgasse 12/II, A-8010 Graz, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Martin Koller
Bioengineering 2022, 9(5), 204; https://doi.org/10.3390/bioengineering9050204
Received: 30 March 2022 / Revised: 29 April 2022 / Accepted: 5 May 2022 / Published: 10 May 2022
(This article belongs to the Special Issue Advances in Polyhydroxyalkanoate (PHA) Production, Volume 3)
Aerobic, hydrogen oxidizing bacteria are capable of efficient, non-phototrophic CO2 assimilation, using H2 as a reducing agent. The presence of explosive gas mixtures requires strict safety measures for bioreactor and process design. Here, we report a simplified, reproducible, and safe cultivation method to produce Cupriavidus necator H16 on a gram scale. Conditions for long-term strain maintenance and mineral media composition were optimized. Cultivations on the gaseous substrates H2, O2, and CO2 were accomplished in an explosion-proof bioreactor situated in a strong, grounded fume hood. Cells grew under O2 control and H2 and CO2 excess. The starting gas mixture was H2:CO2:O2 in a ratio of 85:10:2 (partial pressure of O2 0.02 atm). Dissolved oxygen was measured online and was kept below 1.6 mg/L by a stepwise increase of the O2 supply. Use of gas compositions within the explosion limits of oxyhydrogen facilitated production of 13.1 ± 0.4 g/L total biomass (gram cell dry mass) with a content of 79 ± 2% poly-(R)-3-hydroxybutyrate in a simple cultivation set-up with dissolved oxygen as the single controlled parameter. Approximately 98% of the obtained PHB was formed from CO2. View Full-Text
Keywords: non-phototrophic CO2 assimilation; Knallgas cultivation; Chemolithotrophs; ATEX compliant bioreactor; dissolved oxygen control non-phototrophic CO2 assimilation; Knallgas cultivation; Chemolithotrophs; ATEX compliant bioreactor; dissolved oxygen control
Show Figures

Graphical abstract

MDPI and ACS Style

Lambauer, V.; Kratzer, R. Lab-Scale Cultivation of Cupriavidus necator on Explosive Gas Mixtures: Carbon Dioxide Fixation into Polyhydroxybutyrate. Bioengineering 2022, 9, 204. https://doi.org/10.3390/bioengineering9050204

AMA Style

Lambauer V, Kratzer R. Lab-Scale Cultivation of Cupriavidus necator on Explosive Gas Mixtures: Carbon Dioxide Fixation into Polyhydroxybutyrate. Bioengineering. 2022; 9(5):204. https://doi.org/10.3390/bioengineering9050204

Chicago/Turabian Style

Lambauer, Vera, and Regina Kratzer. 2022. "Lab-Scale Cultivation of Cupriavidus necator on Explosive Gas Mixtures: Carbon Dioxide Fixation into Polyhydroxybutyrate" Bioengineering 9, no. 5: 204. https://doi.org/10.3390/bioengineering9050204

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop