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Article

Use of Stacked Layers of Electrospun L-Lactide/Glycolide Co-Polymer Fibers for Rapid Construction of Skin Sheets

1
Department of Anatomy and Medical Imaging, University of Auckland, Auckland 1023, New Zealand
2
Electrospinz Limited, 44 Lee Street, Blenheim 7201, New Zealand
3
Canterbury Agriculture & Science Centre, The NZ Institute for Plant & Food Research Ltd. Lincoln, Gerald Street, Lincoln 7608, New Zealand
*
Author to whom correspondence should be addressed.
Current address: School of Engineering, University of Lincoln, Brayford Pool, Lincolnshire LN6 7TS, UK.
Bioengineering 2021, 8(1), 7; https://doi.org/10.3390/bioengineering8010007
Received: 20 November 2020 / Revised: 20 December 2020 / Accepted: 4 January 2021 / Published: 7 January 2021
(This article belongs to the Special Issue Electrospinning for Tissue Engineering)
This paper describes a novel method for the rapid construction of skin, using multiple layers of aligned electrospun fibers as starting scaffolds. Scaffolds were spun from biodegradable L-lactide/glycolide (molar ratio 10:90) with predominantly parallel arrays of fibers attached peripherally to thin 304 stainless steel layer frames. Each layer frame was held between two thicker support frames. Human skin cells were seeded onto multiple (three–nine) scaffolds. Dermal fibroblasts were seeded on both sides of each scaffold except for one on which keratinocytes were seeded on one side only. Following 48 h of culture, the scaffolds and layer frames were unmounted from their support frames, stacked, with keratinocytes uppermost, and securely held in place by upper and lower support frames to instantly form a multilayered “dermis” and a nascent epidermis. The stack was cultured for a further 5 days during which time the cells proliferated and then adhered to form, in association with the spun fibers, a mechanically coherent tissue. Fibroblasts preferentially elongated in the dominant fiber direction and a two-dimensional weave of alternating fiber and cell alignments could be constructed by selected placement of the layer frames during stacking. Histology of the 7-day tissue stacks showed the organized layers of fibroblasts and keratinocytes immuno-positive for keratin. Electron microscopy showed attachment of fibroblasts to the lactide/glycolide fibers and small-diameter collagen fibers in the extracellular space. This novel approach could be used to engineer a range of tissues for grafting where rapid construction of tissues with aligned or woven layers would be beneficial. View Full-Text
Keywords: electrospun mesh; layered scaffolds; culture; fibroblasts; keratinocytes; skin electrospun mesh; layered scaffolds; culture; fibroblasts; keratinocytes; skin
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MDPI and ACS Style

Merrilees, M.; Buunk, N.; Zuo, N.; Larsen, N.; Karimi, S.; Tucker, N. Use of Stacked Layers of Electrospun L-Lactide/Glycolide Co-Polymer Fibers for Rapid Construction of Skin Sheets. Bioengineering 2021, 8, 7. https://doi.org/10.3390/bioengineering8010007

AMA Style

Merrilees M, Buunk N, Zuo N, Larsen N, Karimi S, Tucker N. Use of Stacked Layers of Electrospun L-Lactide/Glycolide Co-Polymer Fibers for Rapid Construction of Skin Sheets. Bioengineering. 2021; 8(1):7. https://doi.org/10.3390/bioengineering8010007

Chicago/Turabian Style

Merrilees, Mervyn, Neil Buunk, Ning Zuo, Nigel Larsen, Samaneh Karimi, and Nick Tucker. 2021. "Use of Stacked Layers of Electrospun L-Lactide/Glycolide Co-Polymer Fibers for Rapid Construction of Skin Sheets" Bioengineering 8, no. 1: 7. https://doi.org/10.3390/bioengineering8010007

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