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Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems

1
Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, Hong Kong 999077, China
2
Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong 999077, China
3
CSIRO Manufacturing, Clayton, Victoria 3168, Australia
4
Australian Regenerative Medicine Institute, Monash University, Victoria 3800, Australia
*
Author to whom correspondence should be addressed.
Bioengineering 2020, 7(2), 47; https://doi.org/10.3390/bioengineering7020047
Received: 29 April 2020 / Revised: 18 May 2020 / Accepted: 22 May 2020 / Published: 25 May 2020
(This article belongs to the Special Issue Cell–Biomaterial Interactions)
A critical shortage of donor livers for treating end-stage liver failure signifies the urgent need for alternative treatment options. Hepatocyte-like cells (HLC) derived from various stem cells represent a promising cell source for hepatocyte transplantation, liver tissue engineering, and development of a bioartificial liver assist device. At present, the protocols of hepatic differentiation of stem cells are optimized based on soluble chemical signals introduced in the culture medium and the HLC produced typically retain an immature phenotype. To promote further hepatic differentiation and maturation, biomaterials can be designed to recapitulate cell–extracellular matrix (ECM) interactions in both 2D and 3D configurations. In this review, we will summarize and compare various 2D and 3D biomaterial systems that have been applied to hepatic differentiation, and highlight their roles in presenting biochemical and physical cues to different stem cell sources. View Full-Text
Keywords: hepatic differentiation; stem cell; biomaterial; decellularized extracellular matrix; stiffness; topography hepatic differentiation; stem cell; biomaterial; decellularized extracellular matrix; stiffness; topography
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MDPI and ACS Style

Zhao, X.; Zhu, Y.; Laslett, A.L.; Chan, H.F. Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems. Bioengineering 2020, 7, 47. https://doi.org/10.3390/bioengineering7020047

AMA Style

Zhao X, Zhu Y, Laslett AL, Chan HF. Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems. Bioengineering. 2020; 7(2):47. https://doi.org/10.3390/bioengineering7020047

Chicago/Turabian Style

Zhao, Xiaoyu, Yanlun Zhu, Andrew L. Laslett, and Hon F. Chan. 2020. "Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems" Bioengineering 7, no. 2: 47. https://doi.org/10.3390/bioengineering7020047

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