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A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons

1
Center for Hybrid Nanostructures, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
2
Department of Physics and Astronomy, University College London, London WC1E 6BT , UK
3
Material Science and Engineering, College of Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Bioengineering 2020, 7(2), 46; https://doi.org/10.3390/bioengineering7020046
Received: 3 May 2020 / Revised: 20 May 2020 / Accepted: 20 May 2020 / Published: 22 May 2020
Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent the biological system in mammals at around 37 °C. In order to better mimic the natural environment in electrophysiological studies, we present a custom-built, temperature-controlled patch clamp platform for upright microscopes, which can easily be adapted to any upright patch clamp setup independently, whether commercially available or home built. Our setup can both cool and heat the platform having only small temperature variations of less than 0.5 °C. We demonstrate our setup with patch clamp measurements at 36 °C on Jurkat T lymphocytes and human induced pluripotent stem cell-derived neurons. Passive membrane parameters and characteristic electrophysiological properties, such as the gating properties of voltage-gated ion channels and the firing of action potentials, are compared to measurements at room temperature. We observe that many processes that are not explicitly considered as temperature dependent show changes with temperature. Thus, we believe in the need of a temperature control in patch clamp measurements if improved physiological conditions are required. Furthermore, we advise researchers to only compare electrophysiological results directly that have been measured at similar temperatures since small variations in cellular properties might be caused by temperature alterations. View Full-Text
Keywords: patch clamping; Jurkat cells; human induced pluripotent stem cell-derived neurons; temperature control; electrophysiology patch clamping; Jurkat cells; human induced pluripotent stem cell-derived neurons; temperature control; electrophysiology
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Harberts, J.; Kusch, M.; O’Sullivan, J.; Zierold, R.; Blick, R.H. A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons. Bioengineering 2020, 7, 46.

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