Next Article in Journal
Up-Flow Anaerobic Sludge Blanket (UASB) Technology for Energy Recovery: A Review on State-of-the-Art and Recent Technological Advances
Next Article in Special Issue
Culture Time Needed to Scale up Infrapatellar Fat Pad Derived Stem Cells for Cartilage Regeneration: A Systematic Review
Previous Article in Journal
Purification and Valorization of Waste Cotton Seed Oil as an Alternative Feedstock for Biodiesel Production
Previous Article in Special Issue
Impact of Dual Cell Co-culture and Cell-conditioned Media on Yield and Function of a Human Olfactory Cell Line for Regenerative Medicine
Article

Ex-Vivo Stimulation of Adipose Stem Cells by Growth Factors and Fibrin-Hydrogel Assisted Delivery Strategies for Treating Nerve Gap-Injuries

1
Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital Basel, University of Basel, Spitalstrasse 21, 4021 Basel, Switzerland
2
Department of Pathology, University Hospital Basel, Hebelstrasse 20, 4021 Basel, Switzerland
3
Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland
4
Department of Biomedicine, University Hospital Basel, Hebelstrasse 20, 4021 Basel, Switzerland
5
Department of Neurosurgery, University Hospital Basel, Spitalstrasse 21, 4021 Basel, Switzerland
*
Author to whom correspondence should be addressed.
Daniel F. Kalbermatten and Srinivas Madduri share the senior authorship.
Bioengineering 2020, 7(2), 42; https://doi.org/10.3390/bioengineering7020042
Received: 9 April 2020 / Revised: 1 May 2020 / Accepted: 3 May 2020 / Published: 5 May 2020
(This article belongs to the Special Issue Stem Cell Bioprocessing and Manufacturing)
Peripheral nerve injuries often result in lifelong disabilities despite advanced surgical interventions, indicating the urgent clinical need for effective therapies. In order to improve the potency of adipose-derived stem cells (ASC) for nerve regeneration, the present study focused primarily on ex-vivo stimulation of ASC by using growth factors, i.e., nerve growth factor (NGF) or vascular endothelial growth factor (VEGF) and secondly on fibrin-hydrogel nerve conduits (FNC) assisted ASC delivery strategies, i.e., intramural vs. intraluminal loading. ASC were stimulated by NGF or VEGF for 3 days and the resulting secretome was subsequently evaluated in an in vitro axonal outgrowth assay. For the animal study, a 10 mm sciatic nerve gap-injury was created in rats and reconstructed using FNC loaded with ASC. Secretome derived from NGF-stimulated ASC promoted significant axonal outgrowth from the DRG-explants in comparison to all other conditions. Thus, NGF-stimulated ASC were further investigated in animals and found to enhance early nerve regeneration as evidenced by the increased number of β-Tubulin III+ axons. Notably, FNC assisted intramural delivery enabled the improvement of ASC’s therapeutic efficacy in comparison to the intraluminal delivery system. Thus, ex-vivo stimulation of ASC by NGF and FNC assisted intramural delivery may offer new options for developing effective therapies. View Full-Text
Keywords: adipose stem cells; neurotrophic factors; growth factors; peripheral nerve injuries; fibrin nerve conduits; hydrogels; stem cells delivery; axonal regeneration; Schwann cells adipose stem cells; neurotrophic factors; growth factors; peripheral nerve injuries; fibrin nerve conduits; hydrogels; stem cells delivery; axonal regeneration; Schwann cells
Show Figures

Graphical abstract

MDPI and ACS Style

Prautsch, K.M.; Degrugillier, L.; Schaefer, D.J.; Guzman, R.; Kalbermatten, D.F.; Madduri, S. Ex-Vivo Stimulation of Adipose Stem Cells by Growth Factors and Fibrin-Hydrogel Assisted Delivery Strategies for Treating Nerve Gap-Injuries. Bioengineering 2020, 7, 42. https://doi.org/10.3390/bioengineering7020042

AMA Style

Prautsch KM, Degrugillier L, Schaefer DJ, Guzman R, Kalbermatten DF, Madduri S. Ex-Vivo Stimulation of Adipose Stem Cells by Growth Factors and Fibrin-Hydrogel Assisted Delivery Strategies for Treating Nerve Gap-Injuries. Bioengineering. 2020; 7(2):42. https://doi.org/10.3390/bioengineering7020042

Chicago/Turabian Style

Prautsch, Katharina M.; Degrugillier, Lucas; Schaefer, Dirk J.; Guzman, Raphael; Kalbermatten, Daniel F.; Madduri, Srinivas. 2020. "Ex-Vivo Stimulation of Adipose Stem Cells by Growth Factors and Fibrin-Hydrogel Assisted Delivery Strategies for Treating Nerve Gap-Injuries" Bioengineering 7, no. 2: 42. https://doi.org/10.3390/bioengineering7020042

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop