Next Article in Journal
Biomechanical Restoration Potential of Pentagalloyl Glucose after Arterial Extracellular Matrix Degeneration
Next Article in Special Issue
Surface Area to Volume Ratio of Electrospun Polydioxanone Templates Regulates the Adsorption of Soluble Proteins from Human Serum
Previous Article in Journal
Sources of Collagen for Biomaterials in Skin Wound Healing
Previous Article in Special Issue
Cell Integration with Electrospun PMMA Nanofibers, Microfibers, Ribbons, and Films: A Microscopy Study
Open AccessArticle

Incorporation of Fibrin Matrix into Electrospun Membranes for Periodontal Wound Healing

1
Department of Periodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23298, USA
2
Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23298, USA
3
Department of Oral Health Promotion and Community Outreach, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23298, USA
4
Department of General Practice, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23298, USA
*
Author to whom correspondence should be addressed.
Bioengineering 2019, 6(3), 57; https://doi.org/10.3390/bioengineering6030057
Received: 31 May 2019 / Revised: 22 June 2019 / Accepted: 25 June 2019 / Published: 30 June 2019
(This article belongs to the Special Issue Designing Tissue Scaffolds with Electrospun Fibers)
Guided tissue regeneration (GTR) aims to regenerate the lost attachment apparatus caused by periodontal disease through the use of a membrane. The goal of this study is to create and characterize a novel hybrid membrane that contains biologically active fibrin matrix within a synthetic polycaprolactone (PCL) electrospun membrane. Three-dimensional fibrin matrices and fibrin-incorporated electrospun membrane were created from fresh frozen plasma by centrifugation in glass vials under three different conditions: 400 g for 12 min, 1450 g for 15 min and 3000 g for 60 min. Half the membranes were crosslinked with 1% genipin. Degradation against trypsin indicated biologic stability while uniaxial tensile testing characterized mechanical properties. Continuous data was analyzed by ANOVA to detect differences between groups (p = 0.05). Fibrin-incorporated electrospun membranes showed statistically significant increase in mechanical properties (elastic modulus, strain at break and energy to break) compared to fibrin matrices. While crosslinking had marginal effects on mechanical properties, it did significantly increase biologic stability against trypsin (p < 0.0001). Lastly, membranes generated at 400 g and 1450 g were superior in mechanical properties and biologic stability compared to those generated at 3000 g. Fibrin-incorporated, crosslinked electrospun PCL membranes generated at lower centrifugation forces offers a novel strategy to generate a potentially superior membrane for GTR procedures. View Full-Text
Keywords: electrospinning; membranes; GTR; fibrin; wound healing; regeneration electrospinning; membranes; GTR; fibrin; wound healing; regeneration
Show Figures

Figure 1

MDPI and ACS Style

Wong, C.; Yoganarasimha, S.; Carrico, C.; Madurantakam, P. Incorporation of Fibrin Matrix into Electrospun Membranes for Periodontal Wound Healing. Bioengineering 2019, 6, 57.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop