Next Article in Journal
Hybrid Approach to State Estimation for Bioprocess Control
Next Article in Special Issue
Cyanobacterial PHA Production—Review of Recent Advances and a Summary of Three Years’ Working Experience Running a Pilot Plant
Previous Article in Journal
Computation of Pacemakers Immunity to 50 Hz Electric Field: Induced Voltages 10 Times Greater in Unipolar Than in Bipolar Detection Mode
Previous Article in Special Issue
Biodegradable Polymeric Substances Produced by a Marine Bacterium from a Surplus Stream of the Biodiesel Industry
Open AccessArticle

The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents

1
School of Chemical Engineering, University of Queensland, St Lucia QLD 4072, Australia
2
Veolia Water Technologies AB—AnoxKaldnes, Klosterängsvägen 11A SE-226 47 Lund, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Martin Koller
Bioengineering 2017, 4(1), 20; https://doi.org/10.3390/bioengineering4010020
Received: 6 January 2017 / Revised: 20 February 2017 / Accepted: 23 February 2017 / Published: 7 March 2017
(This article belongs to the Special Issue Advances in Polyhydroxyalkanoate (PHA) Production)
This paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated. View Full-Text
Keywords: PHA; monomer evolution; mixed culture; modeling; polymer composition; biopolymer PHA; monomer evolution; mixed culture; modeling; polymer composition; biopolymer
Show Figures

Graphical abstract

MDPI and ACS Style

Montano-Herrera, L.; Laycock, B.; Werker, A.; Pratt, S. The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents. Bioengineering 2017, 4, 20.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop