Next Article in Journal
Wearable and Invisible Sensor Design for Eye-Motion Monitoring Based on Ferrofluid and Electromagnetic Sensing Technologies
Next Article in Special Issue
Retrospective Analysis of Responders and Impaired Patients with Knee Osteoarthritis Treated with Two Consecutive Injections of Very Pure Platelet-Rich Plasma (PRP)
Previous Article in Journal
Evidence of Copper Nanoparticles and Poly I:C Modulating Cas9 Interaction and Cleavage of COR (Conserved Omicron RNA)
Previous Article in Special Issue
Standardization of Animal Models and Techniques for Platelet-Rich Fibrin Production: A Narrative Review and Guideline
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Bioengineering
Volume 10
Issue 5
10.3390/bioengineering10050513
bioengineering-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Mechanism of Pulp Regeneration Based on Concentrated Growth Factors Regulating Cell Differentiation

by
Sijing Yu
1,†,
Yi Zheng
1,†,
Qiang Guo
2,
Wenxu Li
1,
Ling Ye
1 and
Bo Gao
1,*
1
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
2
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Bioengineering 2023, 10(5), 513; https://doi.org/10.3390/bioengineering10050513
Submission received: 14 March 2023 / Revised: 20 April 2023 / Accepted: 20 April 2023 / Published: 25 April 2023
(This article belongs to the Special Issue Advances in Autologous PRP Therapy)
Browse Figures
Versions Notes

Abstract

:
Concentrated growth factors (CGF) is the newest generation platelet concentrate product, which has been reported to promote the proliferation and differentiation of human dental pulp cells (hDPCs). However, the effect of liquid phase of CGF (LPCGF) has not been reported. This study was aimed to evaluate the influence of LPCGF on the biological properties of hDPCs, and to explore the in vivo mechanism of dental pulp regeneration based on the hDPCs-LPCGF complex transplantation. It was found that LPCGF could promote the proliferation, migration and odontogenic differentiation of hDPCs, and 25% LPCGF induced the most mineralization nodule formation and the highest DSPP gene expression. The heterotopic transplantation of the hDPCs-LPCGF complex resulted in the formation of regenerative pulp tissue with newly formed dentin, neovascularization and nerve-like tissue. Together, these findings provide key data on the effect of LPCGF on the proliferation, migration, odontogenic/osteogenic differentiation of hDPCs, and the in vivo mechanism of hDPCs-LPCGF complex autologous transplantation in pulp regeneration therapy.

1. Introduction

Caries, odontodysplasia and dental trauma can cause pulp necrosis and affect the root development of young permanent teeth. Due to the wide apical foramen and root canal, and the thin dentin wall easily to be fractured, the root canal preparation and filling in young permanent teeth are quite challenging. How to promote the recovery of the pulp health of young permanent teeth and maintain the continuous development of the root is an important challenge in the current endodontic therapy [1,2].
Regenerative endodontic therapies provide a method to replace diseased pulp tissue with new regenerated pulp tissue based on regenerative medicine and biological tissue engineering technology [3,4,5]. From the perspective of application, pulp regeneration therapy includes pulp revascularization, autologous pulp stem cell replantation and cell homing technique [6]. Human dental pulp stem cells (hDPSCs) are a type of stem cells isolated from pulp tissue, which have multipotential differentiation ability. These cells are important source of stem cells to achieve the repair and reconstruction of pulp tissue and have the potential to form pulp-dentin complex [7,8]. Studies have demonstrated that pulp stem cell transplantation can regenerate pulp-like tissue, including the regeneration of pulp-like tissue in ectopic tooth transplantation models [9,10], as well as the clinical observation of healing of apical lesions, restoration of pulp vitality [11], and histological observation of formation of mineralized fibrous connective tissue similar to pulp-like tissue in the root canal [12,13,14].
Growth factors play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates which contain high levels of growth factors are widely applied in dental pulp regeneration research [15,16,17,18]. Different types of autologous platelet concentrates, including platelet rich plasma (PRP), platelet rich fibrin (PRF) and concentrated growth factor (CGF), can be obtained by the exact method of centrifugation. PRP, as the first generation of autologous platelet concentrates, is prepared by the centrifugation of autologous whole blood together with thrombin and calcium chloride [19]. Due to the addition of exogeneic thrombin and anticoagulant in the preparation process, this technology might cause an immunologic and infectious response, making its use controversial [19,20]. In addition, activation with thrombin prior to implantation has been shown to significantly inhibit osteoinductive activity of PRP [21]. The preparation of PRF does not require the addition of any exogenous material and is more widely used in oral and maxillofacial surgical procedures [22]. CGF is the newest platelet concentrate and is produced in a manner similar to that used to produce PRF but involves different centrifugation speeds. There are significant differences in the amounts of growth factors in PRP, PRF and CGF [23]. PRF and CGF extracts seem to contain compatible or higher levels of platelets and platelet-derived growth factors compared to PRP preparations [24]. Studies have shown that the growth factors in CGF can accelerate the process of wound healing and promote tissue regeneration by stimulating cell proliferation and differentiation and promoting stem cell migration, and CGF has been widely used in clinical practice to promote bone tissue formation and soft tissue healing [24,25,26].
Currently, in vitro study has shown that CGF can promote the proliferation and migration of umbilical vein endothelial cells and the formation of tubular structures [27,28], and another in vitro study has also shown that CGF has a positive effect on the proliferation, migration and differentiation of hDPSCs stimulated by lipopolysaccharide [29]. In potential cell-based tissue engineering, LPCGF is more likely to fully mix and contact with stem cells than CGF, providing stem cells with an extracellular microenvironment that is more conducive to pulp tissue regeneration. However, there have been no reports on the role of LPCGF on promoting hDPCs to form pulp-dentin complex in vivo currently. In our study, we hypothesized that LPCGF contains significant growth factors and might promote pulp tissue regeneration. Then we explored the release and degradation trend of growth factors in autologous CGF and LPCGF, the role of LPCGF on proliferation, migration and differentiation of hDPCs in vitro, and on promoting the formation of heterotopic pulp-like tissue in vivo, so as to provide the mechanism for the clinical application of autologous transplanted hDPCs-LPCGF complex in regenerative endodontic therapy.

2. Materials and Methods

2.1. Isolation and Culture of hDPCs

Healthy, intact and caries-free third molars were collected from 18–22 years old patients in the Oral Surgery Department of West China Hospital of Stomatology of Sichuan University, with informed consent of the patients. The hDPCs were isolated by enzyme digestion according to a previously described method [30]. The separated cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, NY, USA), supplemented with 10% fetal bovine serum (FBS; Gibco, Billings, MT, USA) and 100 U/mL Penicillin-Streptomycin (PS; Hyclone, Logan, UT, USA) in a humidified atmosphere of 5% CO2 at 37 °C. Cells at passage 3 (P3) were used in the following study.

2.2. Conditioned Medium Preparation

This study has been approved by the Medical Ethics Committee of West China College of Stomatology, Sichuan University (Ethics Approval Number: WCHSIRB-D2017-215). The volunteers were aged 18–25 years old, and were confirmed that they did not have any of the following conditions: (1) infectious diseases; (2) blood diseases; (3) history of infection and taking antibiotics in the last 3 months; (4) taking aspirin and other drugs that affect platelet function in the last 3 months; (5) women in pregnancy, lactation and menstruation.

2.2.1. Preparation of CGF Extract

Venous blood were collected using red tube cap samplers (no additives) and green tube cap samplers (containing heparin sodium anticoagulant). Then the tubes were immediately centrifuged in the MediFuge centrifuge (Silfradent, Santa Sofia, Italy) by 30 s acceleration, 2 min at 2700 rpm (600 g), 4 min at 2400 rpm (400 g), 4 min at 2700 rpm (600 g), 3 min at 3000 rpm, and 36 s deceleration [31].
The CGF gel (middle layer) in the red cap tube was washed with sterile PBS, frozen with liquid nitrogen for 5 min, and melted at 37 °C for 5 min, repeated three times. Then the CGF extract was centrifuged and filtered for subsequent ELISA experiment. The LPCGF (middle layer) in the green cap tube was divided into three parts. The first part was directly prepared for CGF conditional medium, the second part was heated by activated plasma albumin gel (APAG) device at 75 °C for 5 min for ELISA experiment, co-culture experiment with hDPCs and transplantation of hDPCs-LPCGF complex in nude mice in vivo.

2.2.2. Preparation of CGF Conditional Medium

Each 1 mL LPCGF was added into 9 mL blank medium for 100% LPCGF. The 100% LPCGF was further diluted with blank medium to obtain 50% and 25% LPCGF. Conditioned media containing three concentrations of LPCGF were completed after being supplemented with 10% FBS and 100 U/mL PS.

2.3. Scanning Electron Microscopy (SEM)

LPCGF and hDPC suspension were mixed and evenly inoculated on the cell sliders for co-culture. After 7 days, the cell slides were fixed with 2.5% glutaraldehyde, dehydrated with ethanol gradient, and dried at critical point. Then cell slides were sprayed with gold coating, and the adhesion of hDPCs to LPCGF fibrin network was observed by SEM (JSM-7500F, Tokyo, Japan).

2.4. Cell Proliferation Assay

The hDPCs were seeded on 96-well plates (Corning, NY, USA) at a density of 2 × 103 cells per well. The cells were divided into control group, 25% LPCGF, 50% LPCGF and 100% LPCGF groups. Cell Counting Kit-8 (Beyotime, Shanghai, China) was used to analyze the cell numbers.

2.5. Cell Migration Assay

The hDPCs were divided into control group (normal medium without FBS), 25%, 50% and 100% LPCGF groups (without FBS), and 2% FBS group. After 1 day of culture, a sterile tip was used to scrape horizontal lines at the bottom of each well. After being observed and photographed using an inverted microscope (Olympus, TH4-200, Tokyo, Japan), the cells were cultured for 12 h and then photographed again.

2.6. Alizarin Red S Staining

The cells were divided into four groups: control group, namely mineralization induction solution group (normal medium containing 10% FBS and 100 U/mL PS added with 10 nmol/L dethasone, 10 mmol/L N-glycerophosphate sodium and 50 mg/L vitamin C), and mineralization induction solution added with 100% LPCGF, 50% LPCGF and 25% LPCGF groups. After 7 and 14 days, the hDPCs were fixed in 4% paraformaldehyde, stained with 0.1% alizarin red S solution (Solarbio, Beijing, China), and the formation of mineralization nodules was photographed.

2.7. Detection of ALP Activity

The grouping was the same as Alizarin red S staining. After 7 days, the hDPCs were fixed in 4% paraformaldehyde, added with alkaline phosphatase staining solution (Beyotime, Shanghai, China), and incubated at room temperature for 30 min. Then the formation of mineralization nodules was observed and photographed.

2.8. Real-Time Quantitative Polymerase Chain Reaction

The mRNA expression levels of dentin sialophosphoprotein (DSPP), Runt-related transcription factor 2 (Runx2) were determined by quantitative polymerase chain reaction (qPCR). Total RNA was isolated from cells using TRIZOL reagent. Then, 1μg of total RNA was reversely transcribed into cDNA using PrimeScript™ Kit (Takara, Maebeshi, Japan). The cDNA samples were then amplified using SYBR Premix Ex TaqTM Kit (Takara, Maebeshi, Japan) according to the manufacturer’s instructions. The relative mRNA expression was determined using the comparative Ct (ΔΔCt) method. The primer sequences for DSPP and Runx2 are shown in Table 1.

2.9. Growth Factor Detection by Enzyme-Linked Immunosorbent Assay (ELISA)

The contents of VEGF, BMP2, PDGF-BB and bFGF were determined quantitatively by enzyme-linked immunosorbent assay (ELISA) at 1 and 7 days. The ELISA kit (SunRedBio, Shanghai, China) was used according to the manufacturer’s instructions.

2.10. In Vivo Transplantation Assay of hDPCs and Concentrated Growth Complex in Nude Mice

The 6-week-old female nude mice were obtained from Experimental Animal Center of Sichuan University. The tooth transplantation models were prepared similar to a previously described method [10]. Eighteen single-rooted teeth were randomly divided into three groups. The root canal was performed to the size of 04/35 with MTwo nickel-titanium instruments (VDW, Munchen, Germany). Then the root part was prepared into an 8 mm-long root model with an opening apical orifice of 2 mm in diameter, and alternately rinsed and vibrated with 1.25% NaClO and 17% EDTA solutions. After autoclave sterilization, the coronal orifice of the root models was sealed with MTA and glass ionomer cement.
The hDPCs were resuspend with a density of 1 × 105 cells/mL using normal medium. The gelatinous LPCGF was mixed with the same volume of cell suspension through a three-way tube. The hDPCs-LPCGF complex or hDPCs suspension were separately injected into different root models, and then apical openings were sealed with absorbable gelatin sponge. Different groups of root models were implanted into the subcutaneous tissue on the back of the nude mice. After two months, the subcutaneously transplanted root models were collected for Micro-CT analysis, hematoxylin-eosin (HE) staining and immunohistochemistry (IHC).

2.11. Micro-CT Analysis

Before the in vivo transplantation, the prepared root models were scanned at a voxel size of 10.0 μm in a microcomputer tomography unit (SCANCO Medical AG, CH-8306 Bruettisellen, Switzerland) at settings of 70 kVp and 200 μA. Two months after the operation, the transplanted root models of each group were scanned with the same parameters. The total volume (TV, including the medullary cavity) and the hard tissue volume (bone volume, BV) of the root models were calculated using data analysis software (SCANCO Medical Evaluation), and the BV/TV values of each root model were obtained. Then the ΔBV/TV values were obtained to indicate the changes in hard tissue volume of the root models after the operation, so as to evaluate whether new mineralization tissue was formed.

2.12. Hematoxylin-Eosin Staining

The postoperative samples were demineralized in EDTA solution and prepared into sections. Then the sections were deparaffinized, rehydrated, and then stained by HE staining reagent according to the manufacturer’s instructions.

2.13. IHC

The primary antibodies used for immunochemistry were DSPP, NESTIN and VEGF (Immunoway, Plano, TX, USA). Secondary antibodies were all purchased from ZenBioScience. The sections were deparaffinized, followed by incubation in trypsin antigen retrieval buffer, sealed with oxidoreductase to suppress endogenous peroxidase activity, and incubated with primary antibody at 4 °C overnight. Then the sections were incubated with secondary antibody for 30 min at 37 °C. The sections were added with color developing agent DAB (DAKO, Glostrup, Denmark) and counterstained with hematoxylin.

2.14. Statistical Analysis

Statistical analysis was performed by using SPSS version 19.0 statistical software. The one-way analysis of variance (ANOVA) test and Tukey’s t-test were used for statistical analysis. Statistical significance was accepted at p < 0.05.

3. Results

3.1. Characteristics of hDPCs

The primary hDPCs exhibited a spindle shape with a single nucleus, and some cells were elliptic or polygonal in scattered distribution. After passage, the hDPCs were mostly spindle-shaped and closely arranged (Figure 1), and multi-layer growth could be observed after single layer overgrowth.

3.2. Adhesion of hDPCs to LPCGF Fibrin Network

After the co-culture of hDPCs and LPCGF for 7 days, hDPCs were growing in a relatively large number and dense distribution. The cells extended many long and thin pseudopods connected to each other to form a laminated network. White blood cells (large and spherical) and red blood cells (disc-shaped and double-concave) were scattered in the interspaces of LPCGF fibrin network (Figure 2).

3.3. Effect of LPCGF on hDPC Proliferation

100%, 50% and 25% LPCGF showed no accelerated effect on hDPC proliferation on day 1 (p > 0.05), and all increased proliferation rate of hDPCs on day 5 (p < 0.05, p < 0.001 and p < 0.0001). Different concentrations of LPCGF could increase cell proliferation in a dose-dependent manner, and 100% LPCGF showed the most obvious promoting effect on the proliferation of hDPCs (Figure 3).

3.4. Effect of LPCGF on hDPC Migration

The effect of 25% and 50% LPCGF on cell migration was greater than that of 100% LPCGF group, and all LPCGF groups showed higher migration ability than that of control group (Figure 4).

3.5. The Effect of LPCGF on hDPC Differentiation

The results of alizarin red S staining (Figure 5A) showed that the osteogenic induction medium with different concentrations of LPCGF could successfully induce osteogenic differentiation and mineralization nodule formation of hDPCs. On day 14, the number and area of mineralization nodules in the LPCGF groups were increased. In particular, the number and area of mineralization nodules in the 25% LPCGF group were higher than those in the other two LPCGF groups. The results of ALP staining (Figure 5B) showed that the LPCGF groups with different concentrations had deeper staining than the control group, indicating that LPCGF could promote the odontogenic differentiation of hDPCs.
DSPP and Runx2 expression in hDPCs after 7 days of mineralization induction were shown (Figure 5C). DSPP expression in the 25% LPCGF group was 1.8-fold higher than that in the control group (p < 0.05), while Runx2 expression in the LPCGF groups with different concentrations had no significant difference from that in the control group (p > 0.05).

3.6. Changes of Growth Factor Content in CGF and LPCGF

The concentrations of bFGF, BMP-2, PDGF and VEGF in the CGF group were higher than those in the heat-treated LPCGF group (p < 0.05). Over time, the BMP-2 release in CGF group showed an upward trend (p < 0.01), while VEGF and bFGF showed a downward trend (p < 0.01), and PDGF-BB showed no significant change. In the LPCGF group, the release of these four growth factors was relatively stable, and no significant changes were observed with time (Figure 6).

3.7. The Effect of hDPCs-LPCGF Complex on the Pulp Tissue Regeneration and the Root/Apical Foramen Development In Vivo

Two months after the transplantation, the nude mice were sacrificed, and new soft tissues were visible in the root canal of the root models (Figure 7A).

3.7.1. Micro-CT Results

Newly formed hard tissue could be observed in the root canal wall of the root models in the hDPCs-LPCGF complex group (Figure 7B), while no obvious new hard tissue was observed in the control group and hDPCs group. Micro-CT analysis showed that the increase of hard tissue in hDPCs-LPCGF group was significantly higher than that in control group and hDPCs group (p < 0.01 and p < 0.05) (Figure 7C).

3.7.2. Histologic Results

HE staining (Figure 8A) showed that in hDPCs-LPCGF group, new pulp-like tissue was observed, including new dentin deposition in the root canal wall, vascular network structure and fibrous tissue in the lumen. In the hDPCs group, there was a small amount of fibrous tissue in the root canal. In the control group, the root canal was empty.
IHC staining (Figure 8B) showed that DSPP positive signals were mostly distributed near the odontoblast cell layer in the medullary cavity, indicating the presence of newly generated dentin. VEGF positive signals were distributed around the vessel wall, suggesting neovascularization tissue. NESTIN positive signals were scattered among the tissue in the medullary cavity, suggesting the formation of nerve-like tissue.

4. Discussion

The preparation of CGF and LPCGF does not require the addition of chemical activators, and different centrifugation rates in the program can be set to allow platelets to collide more adequately, thus releasing more growth factors. CGF contains abundant growth factors, including TGF-β, VEGF, PDGF, bFGF, BMP, insulin-like growth factor (IGF), epidermal growth factor (EGF) and osteoprotegerin (OPG) [24]. CGF also has better biocompatibility and more stable fibrin network structure with excellent mechanical properties of tensile strength and fracture strain, which can avoid the enzymatic hydrolysis of growth factors in the form of chemical bonds and extend its action time [32,33,34]. Furthermore, CGF contains a denser and richer GF–fibrin matrix in which growth factors are closely bound to one another [23]. This provides the slow release of growth factors, which helps with wound healing [20,35].
As a product of improved CGF technology, LPCGF exhibited positive promoting effects on hDPC proliferation, migration and dentinogenic differentiation. The results of our study showed that LPCGF promoted the proliferation of hDPCs in a concentration- and time-dependent manner, and enhanced the migration of hDPCs. It was possibly because LPCGF contained a large number of growth factors, such as BMP-2, bFGF, VEGF and PDGF, which were proved to promote the migration, proliferation and angiogenic differentiation of hDPSCs [36,37,38,39,40]. BMP-2 is a powerful osteoinductive cytokine which can induce the differentiation of undifferentiated mesenchymal cells into cartilage and new bone. The fusion of growth factor BMP-2 into the tissue engineering scaffold structure can not only improve the interface contact between hDPSCs and the scaffold, but also make the chemical composition of the material similar to the extracellular matrix, which is conducive to the growth of hDPSCs [40]. FGF-2 could activate HDPC growth and migration [36], and has an anti-apoptotic effect on fibroblasts [41]. VEGF and FGF-2 were both proved to enhance dental pulp regeneration and neovascularization [37,38]. PDGF is an effective growth factor for wound healing [39] and has the effect of stabilizing neovascularization [42]. PDGF can also promote the proliferation and recruitment of periodontal osteocytes and achieve periodontal regeneration [43]. It has also been proved that PDGF could significantly enhance hDPSC proliferation [44].
In addition, our study indicated that LPCGF could promote the dentinogenic differentiation of hDPCs. TGF-β can promote the differentiation of hDPCs into odontoblast stem cells and up-regulate the matrix secretion of the dentin-pulp complex [45]. TGF-β can also significantly increase the proliferation and ALPase activity of human dental pulp fibroblasts, which could then aggregate to form mineralization nodules [46]. BMP-7 has been reported to promote the odontogenic differentiation and mineralization of hDPSCs [47]. Interestingly, LPCGF induced the DSPP expression of hDPCs, while it had no effect on Runx2 expression. DSPP is positively expressed in the early odontoblastic differentiation of hDPSCs [48], and Runx2 is an early regulator of osteoblast differentiation [49], which indicated that under the stimulation of LPCGF, the hDPCs were more inclined to odontogenic differentiation rather than osteogenic differentiation, and made it a more suitable material for dentin-pulp complex regeneration.
BMP-2, bFGF, VEGF and PDGF-BB were quantitatively detected to investigate the difference in the release concentration in CGF and heat-treated LPCGF, and the results confirmed that both CGF and LPCGF contained these growth factors. Our results have also suggected that 25% LPCGF promoted hDPC mineralization most significantly. Therefore, although LPCGF contained less growth factors than CGF, it might play a better role in promoting odontogenic differentiation of hDPCs. And the slow release and stable concentration range of growth factors of LPCGF were superior to CGF, which ensured that sufficient local concentration of growth factors could be obtained in the following heterotopic transplantation of hDPCs-LPCGF complex in vivo.
Autologous dental pulp stem cells have great odontogenic differentiation potential, convenient access and extensive sources [7], which have a good research prospect in dental pulp regeneration through autologous stem cell transplantation. A clinical study has demonstrated the therapeutic potential of mobilized dental pulp stem cells for complete pulp regeneration [11]. Our study further revealed the potential mechanism of hDPC transplantation achieving pulp regeneration. Our results indicated that the hDPCs-LPCGF complex could promote the deposition of mineralization tissue in the root canal wall in vivo, and might continuously thicken the dentin wall after implantation. And the histologic results showed that the hDPCs-LPCGF complex could successfully regenerate dentin-pulp complex structure heterotropically. The regenerated pulp tissue, including neovascularization and nerve-like tissue, almost filled the middle and apical part of the root canal, and there was new secondary dentin deposition in the root canal wall. In hDPCs group, there was only a small amount of newly formed pulp tissue in the root canal, probably because the cell suspension was fluid without scaffold structure, thus it could not be anastomotic with host blood vessels to obtain sufficient nutritional supply, resulting in the death of the implanted stem cells [50]. The heating treatment of LPCGF turned it into a gelatinous structure, which provided a more stable scaffold for cell colonization and growth. In addition, LPCGF is easier to fully mix and contact with stem cells compared to CGF.
Overall, our research showed that The co-culture system of hDPCs and LPCGF could form a stable three-dimensional porous structure containing platelets, white blood cells and growth factors, which could make hDPCs easier to migration, adhesion, proliferation and differentiation, and the hDPCs were more inclined to odontogenic differentiation which was more suitable for dentin-pulp complex regeneration; the hDPCs-LPCGF complex is holo autogenous, and the LPCGF is degradable and can be gradually replaced by regenerated pulp tissue without residual exogenous tissue; the LPCGF can provide and persistently release various growth factors and maintain relatively stable growth factor concentration in the local microenvironment for dental pulp regeneration. Therefore, hDPCs-LPCGF complex is expected to be a feasible method for the study of pulp regeneration therapy.

5. Conclusions

In summary, our in vitro results shed light on the potential of LPCGF on promoting the proliferation, migration and differentiation of hDPCs, and the in vivo results proved that hDPCs-LPCGF complex could heterotropically regenerate dental pulp tissue with newly formed dentin, neovascularization and nerve-like tissue, which provides a potential in vivo mechanism of the hDPCs-LPCGF complex transplantation promoting dental pulp regeneration. However, due to the fact that LPCGF is a mixture with fibrin, growth factors, and various cells, future research may focus on the impact of a single component of LPCGF on the regeneration of the pulp-dentin complex, thereby further revealing the potential mechanism. At the same time, more randomized clinical controlled trials are needed to verify the role of hDPCs-LPCGF complex transplantation in regenerative endodontic therapy of pulpitis and periapical periodontitis of young permanent teeth.

6. Patents

The preparation method of hDPCs-LPCGF complex resulting from the work reported in this manuscript has been authorized for invention patent (Patent Number: CN113975467A).

Author Contributions

Conceptualization: B.G.; Methodology: S.Y. and Y.Z.; Investigation: Y.Z., S.Y. and W.L.; Formal analysis: Y.Z.; Writing-original draft preparation: S.Y., Y.Z. and W.L.; Writing-review and editing: L.Y. and B.G.; Visualization: Q.G. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Sichuan Science and Technology Program (2023NSFSC0575) and Chengdu Key Application Projects of Science and Technology (2021-YF09-00078-SN).

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki and approved by the Medical Ethics Committee of West China College of Stomatology, Sichuan University (Ethics Approval Number: WCHSIRB-D-2017-215, 20 December 2017). The animal study protocol was approved by the Medical Ethics Committee of West China College of Stomatology, Sichuan University (Ethics Approval Number: WCHSIRB-D-2017-194, 2017-12-20).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Conflicts of Interest

The authors deny any conflicts of interest related to this study.

References

  1. Diogenes, A.; Ruparel, N.B.; Shiloah, Y.; Hargreaves, K.M. Regenerative endodontics: A way forward. J. Am. Dent. Assoc. 2016, 147, 372–380. [Google Scholar] [CrossRef] [PubMed]
  2. Kim, S.G.; Malek, M.; Sigurdsson, A.; Lin, L.M.; Kahler, B. Regenerative endodontics: A comprehensive review. Int. Endod. J. 2018, 51, 1367–1388. [Google Scholar] [CrossRef] [PubMed]
  3. Morotomi, T.; Washio, A.; Kitamura, C. Current and future options for dental pulp therapy. Jpn. Dent. Sci. Rev. 2019, 55, 5–11. [Google Scholar] [CrossRef] [PubMed]
  4. Murray, P.E.; Garcia-Godoy, F.; Hargreaves, K.M. Regenerative endodontics: A review of current status and a call for action. J. Endod. 2007, 33, 377–390. [Google Scholar] [CrossRef]
  5. Nakashima, M.; Akamine, A. The application of tissue engineering to regeneration of pulp and dentin in endodontics. J. Endod. 2005, 31, 711–718. [Google Scholar] [CrossRef]
  6. Huang, D.M.; Yang, M.B.; Zhou, X.D. Clinical management and prognosis evaluation of pulp regeneration therapy. Zhonghua Kou Qiang Yi Xue Za Zhi 2019, 54, 584–590. [Google Scholar]
  7. Gronthos, S.; Mankani, M.; Brahim, J.; Robey, P.G.; Shi, S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc. Natl. Acad. Sci. USA 2000, 97, 13625–13630. [Google Scholar] [CrossRef]
  8. Bar, J.K.; Lis-Nawara, A.; Grelewski, P.G. Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential. Int. J. Mol. Sci. 2021, 22, 12018. [Google Scholar] [CrossRef]
  9. Nakayama, H.; Iohara, K.; Hayashi, Y.; Okuwa, Y.; Kurita, K.; Nakashima, M. Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth. Oral Dis. 2017, 23, 620–628. [Google Scholar] [CrossRef]
  10. Murakami, M.; Horibe, H.; Iohara, K.; Hayashi, Y.; Osako, Y.; Takei, Y.; Nakata, K.; Motoyama, N.; Kurita, K.; Nakashima, M. The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential. Biomaterials 2013, 34, 9036–9047. [Google Scholar] [CrossRef]
  11. Nakashima, M.; Iohara, K.; Murakami, M.; Nakamura, H.; Sato, Y.; Ariji, Y.; Matsushita, K. Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: A pilot clinical study. Stem Cell Res. Ther. 2017, 8, 61. [Google Scholar] [CrossRef]
  12. Shimizu, E.; Ricucci, D.; Albert, J.; Alobaid, A.S.; Gibbs, J.L.; Huang, G.T.-J.; Lin, L.M. Clinical, radiographic, and histological observation of a human immature permanent tooth with chronic apical abscess after revitalization treatment. J. Endod. 2013, 39, 1078–1083. [Google Scholar] [CrossRef]
  13. Becerra, P.; Ricucci, D.; Loghin, S.; Gibbs, J.L.; Lin, L.M. Histologic study of a human immature permanent premolar with chronic apical abscess after revascularization/revitalization. J. Endod. 2014, 40, 133–139. [Google Scholar] [CrossRef]
  14. Martin, G.; Ricucci, D.; Gibbs, J.L.; Lin, L.M. Histological findings of revascularized/revitalized immature permanent molar with apical periodontitis using platelet-rich plasma. J. Endod. 2013, 39, 138–144. [Google Scholar] [CrossRef]
  15. Xu, J.; Gou, L.; Zhang, P.; Li, H.; Qiu, S. Platelet-rich plasma and regenerative dentistry. Aust. Dent. J. 2020, 65, 131–142. [Google Scholar] [CrossRef]
  16. Panda, S.; Mishra, L.; Arbildo-Vega, H.I.; Lapinska, B.; Lukomska-Szymanska, M.; Khijmatgar, S.; Parolia, A.; Bucchi, C.; Del Fabbro, M. Effectiveness of Autologous Platelet Concentrates in Management of Young Immature Necrotic Permanent Teeth—A Systematic Review and Meta-Analysis. Cells 2020, 9, 2241. [Google Scholar] [CrossRef]
  17. Arshad, S.; Tehreem, F.; Khan, M.R.; Ahmed, F.; Marya, A.; Karobari, M.I. Platelet-Rich Fibrin Used in Regenerative Endodontics and Dentistry: Current Uses, Limitations, and Future Recommendations for Application. Int. J. Dent. 2021, 2021, 4514598. [Google Scholar] [CrossRef]
  18. Del Fabbro, M.; Lolato, A.; Bucchi, C.; Taschieri, S.; Weinstein, R.L. Autologous Platelet Concentrates for Pulp and Dentin Regeneration: A Literature Review of Animal Studies. J. Endod. 2016, 42, 250–257. [Google Scholar] [CrossRef]
  19. Whitman, D.H.; Berry, R.L.; Green, D.M. Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J. Oral Maxillofac. Surg. 1997, 55, 1294–1299. [Google Scholar] [CrossRef]
  20. Fang, D.; Long, Z.; Hou, J. Clinical Application of Concentrated Growth Factor Fibrin Combined with Bone Repair Materials in Jaw Defects. J. Oral Maxillofac. Surg. 2020, 78, 882–892. [Google Scholar] [CrossRef]
  21. Harrison, S.; Vavken, P.; Kevy, S.; Jacobson, M.; Zurakowski, D.; Murray, M.M. Platelet activation by collagen provides sustained release of anabolic cytokines. Am. J. Sports Med. 2011, 39, 729–734. [Google Scholar] [CrossRef] [PubMed]
  22. Dohan, D.M.; Choukroun, J.; Diss, A.; Dohan, S.L.; Dohan, A.J.; Mouhyi, J.; Gogly, B. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2006, 101, e37–e44. [Google Scholar] [CrossRef] [PubMed]
  23. Mijiritsky, E.; Assaf, H.D.; Peleg, O.; Shacham, M.; Cerroni, L.; Mangani, L. Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review. Biology 2021, 10, 317. [Google Scholar] [CrossRef] [PubMed]
  24. Masuki, H.; Okudera, T.; Watanabe, T.; Suzuki, M.; Nishiyama, K.; Okudera, H.; Nakata, K.; Uematsu, K.; Su, C.-Y.; Kawase, T. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int. J. Implant. Dent. 2016, 2, 19. [Google Scholar] [CrossRef]
  25. Tabatabaei, F.; Aghamohammadi, Z.; Tayebi, L. In vitro and in vivo effects of concentrated growth factor on cells and tissues. J. Biomed. Mater. Res. A 2020, 108, 1338–1350. [Google Scholar] [CrossRef]
  26. Takeda, Y.; Katsutoshi, K.; Matsuzaka, K.; Inoue, T. The Effect of Concentrated Growth Factor on Rat Bone Marrow Cells In Vitro and on Calvarial Bone Healing In Vivo. Int. J. Oral Maxillofac. Implants 2015, 30, 1187–1196. [Google Scholar] [CrossRef]
  27. Jun, H.; Lei, D.; Qifang, Y.; Yuan, X.; Deqin, Y. Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: An in vitro study. Braz. Oral Res. 2018, 32, e48. [Google Scholar] [CrossRef]
  28. Pirpir, C.; Yilmaz, O.; Candirli, C.; Balaban, E. Evaluation of effectiveness of concentrated growth factor on osseointegration. Int. J. Implant Dent. 2017, 3, 7. [Google Scholar] [CrossRef]
  29. Xu, F.; Qiao, L.; Zhao, Y.; Chen, W.; Hong, S.; Pan, J.; Jiang, B. The potential application of concentrated growth factor in pulp regeneration: An in vitro and in vivo study. Stem Cell Res. Ther. 2019, 10, 134. [Google Scholar] [CrossRef]
  30. Sun, H.-H.; Chen, B.; Zhu, Q.-L.; Kong, H.; Li, Q.-H.; Gao, L.-N.; Xiao, M.; Chen, F.-M.; Yu, Q. Investigation of dental pulp stem cells isolated from discarded human teeth extracted due to aggressive periodontitis. Biomaterials 2014, 35, 9459–9472. [Google Scholar] [CrossRef]
  31. Honda, H.; Tamai, N.; Naka, N.; Yoshikawa, H.; Myoui, A. Bone tissue engineering with bone marrow-derived stromal cells integrated with concentrated growth factor in Rattus norvegicus calvaria defect model. J. Artif. Organs 2013, 16, 305–315. [Google Scholar] [CrossRef]
  32. Rodella, L.F.; Favero, G.; Boninsegna, R.; Buffoli, B.; Labanca, M.; Scarì, G.; Sacco, L.; Batani, T.; Rezzani, R. Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction. Microsc. Res. Tech. 2011, 74, 772–777. [Google Scholar] [CrossRef]
  33. Park, H.C.; Kim, S.G.; Oh, J.S.; You, J.S.; Kim, J.S.; Lim, S.C.; Jeong, M.-A.; Kim, J.-S.; Jung, C.; Kwon, Y.-S.; et al. Early Bone Formation at a Femur Defect Using CGF and PRF Grafts in Adult Dogs: A Comparative Study. Implant Dent. 2016, 25, 387–393. [Google Scholar] [CrossRef]
  34. Isobe, K.; Watanebe, T.; Kawabata, H.; Kitamura, Y.; Okudera, T.; Okudera, H.; Uematsu, K.; Okuda, K.; Nakata, K.; Tanaka, T.; et al. Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF). Int. J. Implant Dent. 2017, 3, 17. [Google Scholar] [CrossRef]
  35. Wang, L.; Wan, M.; Li, Z.; Zhong, N.; Liang, D.; Ge, L. A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro. Mol. Med. Rep. 2019, 20, 1039–1048. [Google Scholar] [CrossRef]
  36. Shimabukuro, Y.; Ueda, M.; Ozasa, M.; Anzai, J.; Takedachi, M.; Yanagita, M.; Ito, M.; Hashikawa, T.; Yamada, S.; Murakami, S. Fibroblast growth factor-2 regulates the cell function of human dental pulp cells. J. Endod. 2009, 35, 1529–1535. [Google Scholar] [CrossRef]
  37. Mullane, E.M.; Dong, Z.; Sedgley, C.M.; Hu, J.C.; Botero, T.M.; Holland, G.R.; Nör, J.E. Effects of VEGF and FGF2 on the revascularization of severed human dental pulps. J. Dent. Res. 2008, 87, 1144–1148. [Google Scholar] [CrossRef]
  38. Li, X.W.; Sun, H.C.; Liu, X.H. Vascular endothelial growth factor-loaded microspheres promote dental pulp regeneration and vascularization. Zhonghua Kou Qiang Yi Xue Za Zhi 2018, 53, 42–48. [Google Scholar]
  39. Pierce, G.F.; Mustoe, T.A.; Altrock, B.W.; Deuel, T.F.; Thomason, A. Role of platelet-derived growth factor in wound healing. J. Cell Biochem. 1991, 45, 319–326. [Google Scholar] [CrossRef]
  40. Conde, M.C.; Chisini, L.A.; Demarco, F.F.; Nor, J.E.; Casagrande, L.; Tarquinio, S.B. Stem cell-based pulp tissue engineering: Variables enrolled in translation from the bench to the bedside, a systematic review of literature. Int. Endod. J. 2016, 49, 543–550. [Google Scholar] [CrossRef]
  41. Akasaka, Y.; Ono, I.; Kamiya, T.; Ishikawa, Y.; Kinoshita, T.; Ishiguro, S.; Ishiguro, S.; Yokoo, T.; Imaizumi, R.; Inomata, N.; et al. The mechanisms underlying fibroblast apoptosis regulated by growth factors during wound healing. J. Pathol. 2010, 221, 285–299. [Google Scholar] [CrossRef] [PubMed]
  42. Lachapelle, F.; Avellana-Adalid, V.; Nait-Oumesmar, B.; Baron-Van Evercooren, A. Fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor AB (PDGF AB) promote adult SVZ-derived oligodendrogenesis in vivo. Mol. Cell Neurosci. 2002, 20, 390–403. [Google Scholar] [CrossRef]
  43. Nevins, M.; Camelo, M.; Nevins, M.L.; Schenk, R.K.; Lynch, S.E. Periodontal regeneration in humans using recombinant human platelet-derived growth factor-BB (rhPDGF-BB) and allogenic bone. J. Periodontol. 2003, 74, 1282–1292. [Google Scholar] [CrossRef] [PubMed]
  44. Zhang, M.; Jiang, F.; Zhang, X.; Wang, S.; Jin, Y.; Zhang, W.; Jiang, X. The Effects of Platelet-Derived Growth Factor-BB on Human Dental Pulp Stem Cells Mediated Dentin-Pulp Complex Regeneration. Stem Cells Transl. Med. 2017, 6, 2126–2134. [Google Scholar] [CrossRef] [PubMed]
  45. Dobie, K.; Smith, G.; Sloan, A.J.; Smith, A.J. Effects of alginate hydrogels and TGF-beta 1 on human dental pulp repair in vitro. Connect. Tissue Res. 2002, 43, 387–390. [Google Scholar] [CrossRef]
  46. Nie, X.; Tian, W.; Zhang, Y.; Chen, X.; Dong, R.; Jiang, M.; Chen, F.; Jin, Y. Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns. Cell Biol. Int. 2006, 30, 295–300. [Google Scholar] [CrossRef]
  47. Zhu, L.; Ma, J.; Mu, R.; Zhu, R.; Chen, F.; Wei, X.; Shi, X.; Zang, S.; Jin, L. Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro. Life Sci. 2018, 202, 175–181. [Google Scholar] [CrossRef]
  48. Qin, C.; Brunn, J.; Cadena, E.; Ridall, A.; Tsujigiwa, H.; Nagatsuka, H.; Nagai, N.; Butler, W. The expression of dentin sialophosphoprotein gene in bone. J. Dent. Res. 2002, 81, 392–394. [Google Scholar] [CrossRef]
  49. Franceschi, R.T.; Xiao, G. Regulation of the osteoblast-specific transcription factor, Runx2: Responsiveness to multiple signal transduction pathways. J. Cell Biochem. 2003, 88, 446–454. [Google Scholar] [CrossRef]
  50. Brazelton, T.R.; Blau, H.M. Optimizing techniques for tracking transplanted stem cells in vivo. Stem Cells 2005, 23, 1251–1265. [Google Scholar] [CrossRef]
Bioengineering 10 00513 g001 550
Figure 1. The characteristics of hDPCs: (A) The primary hDPCs; (B) the cells after passage. Scale bar = 200 μm.
Figure 1. The characteristics of hDPCs: (A) The primary hDPCs; (B) the cells after passage. Scale bar = 200 μm.
Bioengineering 10 00513 g001
Bioengineering 10 00513 g002 550
Figure 2. Adhesion of hDPCS to LPCGF fibrin network. (A) Yellow arrowhead indicates hDPCs, and red arrowhead indicates fibrin network; (B) Yellow arrowhead indicates white blood cells, and red arrowhead indicates red blood cells.
Figure 2. Adhesion of hDPCS to LPCGF fibrin network. (A) Yellow arrowhead indicates hDPCs, and red arrowhead indicates fibrin network; (B) Yellow arrowhead indicates white blood cells, and red arrowhead indicates red blood cells.
Bioengineering 10 00513 g002
Bioengineering 10 00513 g003 550
Figure 3. Effect of LPCGF on hDPC proliferation for 1, 3 and 5 days. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Figure 3. Effect of LPCGF on hDPC proliferation for 1, 3 and 5 days. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Bioengineering 10 00513 g003
Bioengineering 10 00513 g004 550
Figure 4. Effect of LPCGF on hDPC migration for 12 h.
Figure 4. Effect of LPCGF on hDPC migration for 12 h.
Bioengineering 10 00513 g004
Bioengineering 10 00513 g005 550
Figure 5. The effect of LPCGF on hDPC mineralization and differentiation. (A) The effects of LPCGF on alizarin red staining in hDPCs after culture for 7 days and 14 days. (B) The effects of LPCGF on ALP staining in hDPCs after culture for 7 days. (C) Relative gene expression levels of DSPP and Runx2 of the hDPCs after culture for 7 days. (* p < 0.05).
Figure 5. The effect of LPCGF on hDPC mineralization and differentiation. (A) The effects of LPCGF on alizarin red staining in hDPCs after culture for 7 days and 14 days. (B) The effects of LPCGF on ALP staining in hDPCs after culture for 7 days. (C) Relative gene expression levels of DSPP and Runx2 of the hDPCs after culture for 7 days. (* p < 0.05).
Bioengineering 10 00513 g005
Bioengineering 10 00513 g006 550
Figure 6. The concentrations of bFGF, BMP-2, PDGF and VEGE in CGF and LPCGF. (** p < 0.01, *** p < 0.001, **** p < 0.0001).
Figure 6. The concentrations of bFGF, BMP-2, PDGF and VEGE in CGF and LPCGF. (** p < 0.01, *** p < 0.001, **** p < 0.0001).
Bioengineering 10 00513 g006
Bioengineering 10 00513 g007 550
Figure 7. The gross and micro-CT results of hDPCs-LPCGF complex on the pulp tissue regeneration in vivo. (A) New soft tissues were visible in the root canal of the root models after 2 months of hDPCs-LPCGF transplantation. (B) The preoperative (a) and postoperative (b) cross sections of the root models in hDPCs-LPCGF complex group. (C) The increase of hard tissue in hDPCs-LPCGF, hDPCs and control groups. (* p < 0.05, ** p < 0.01).
Figure 7. The gross and micro-CT results of hDPCs-LPCGF complex on the pulp tissue regeneration in vivo. (A) New soft tissues were visible in the root canal of the root models after 2 months of hDPCs-LPCGF transplantation. (B) The preoperative (a) and postoperative (b) cross sections of the root models in hDPCs-LPCGF complex group. (C) The increase of hard tissue in hDPCs-LPCGF, hDPCs and control groups. (* p < 0.05, ** p < 0.01).
Bioengineering 10 00513 g007
Bioengineering 10 00513 g008 550
Figure 8. The histologic results of hDPCs-LPCGF complex on pulp regeneration and root/apical foramen development in vivo. (A) The HE staining results of the hDPCs-LPCGF, hDPCs and control groups. (B) The IHC staining results of DSPP, VEGF and NESTIN in the hDPCs-LPCGF, hDPCs and control groups, and the magnification is 40×. Black arrowheads indicate the intense positive staining. Scale bar = 50 μm.
Figure 8. The histologic results of hDPCs-LPCGF complex on pulp regeneration and root/apical foramen development in vivo. (A) The HE staining results of the hDPCs-LPCGF, hDPCs and control groups. (B) The IHC staining results of DSPP, VEGF and NESTIN in the hDPCs-LPCGF, hDPCs and control groups, and the magnification is 40×. Black arrowheads indicate the intense positive staining. Scale bar = 50 μm.
Bioengineering 10 00513 g008
Table
Table 1. Primer sequences used in the real-time PCR.
Table 1. Primer sequences used in the real-time PCR.
GenePrimer Sequences
GAPDH-HUMAN-FGGAGCGAGATCCCTCCAAAAT
GAPDH-HUMAN-RGGCTGTTGTCATACTTCTCATGG
DSPP-FTTTGGGCAGTAGCATGGGC
DSPP-RCCATCTTGGGTATTCTCTTGCCT
RUNX2-FCCTTTACTTACACCCCGCCA
RUNX2-RGGATCCTGACGAAGTGCCAT
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Yu, S.; Zheng, Y.; Guo, Q.; Li, W.; Ye, L.; Gao, B. Mechanism of Pulp Regeneration Based on Concentrated Growth Factors Regulating Cell Differentiation. Bioengineering 2023, 10, 513. https://doi.org/10.3390/bioengineering10050513

AMA Style

Yu S, Zheng Y, Guo Q, Li W, Ye L, Gao B. Mechanism of Pulp Regeneration Based on Concentrated Growth Factors Regulating Cell Differentiation. Bioengineering. 2023; 10(5):513. https://doi.org/10.3390/bioengineering10050513

Chicago/Turabian Style

Yu, Sijing, Yi Zheng, Qiang Guo, Wenxu Li, Ling Ye, and Bo Gao. 2023. "Mechanism of Pulp Regeneration Based on Concentrated Growth Factors Regulating Cell Differentiation" Bioengineering 10, no. 5: 513. https://doi.org/10.3390/bioengineering10050513

APA Style

Yu, S., Zheng, Y., Guo, Q., Li, W., Ye, L., & Gao, B. (2023). Mechanism of Pulp Regeneration Based on Concentrated Growth Factors Regulating Cell Differentiation. Bioengineering, 10(5), 513. https://doi.org/10.3390/bioengineering10050513

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop