Next Article in Journal
Clinical Benefit of Autologous Platelet-Rich Plasma Infusion in Ovarian Function Rejuvenation: Evidence from a Before-After Prospective Pilot Study
Previous Article in Journal
Effect of Low- and High-Frequency Auricular Stimulation with Electro-Acupuncture on Cutaneous Microcirculation: A Cross-Over Study in Healthy Subjects
Previous Article in Special Issue
DNA Damage as a Mechanistic Link between Air Pollution and Obesity?
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Medicines
Volume 10
Issue 2
10.3390/medicines10020018
medicines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Fasting Protocols Do Not Improve Intestinal Architecture and Immune Parameters in C57BL/6 Male Mice Fed a High Fat Diet

by
Raed Y. Ageeli
,
Sunita Sharma
,
Melissa Puppa
,
Richard J. Bloomer
,
Randal K. Buddington
and
Marie van der Merwe
*
College of Health Sciences, The University of Memphis, Memphis, TN 38152, USA
*
Author to whom correspondence should be addressed.
Medicines 2023, 10(2), 18; https://doi.org/10.3390/medicines10020018
Submission received: 23 December 2022 / Revised: 8 February 2023 / Accepted: 9 February 2023 / Published: 17 February 2023
(This article belongs to the Special Issue Perspective of Metabolism: Potential Therapeutic Targets of Metabolic Diseases such as Obesity-Associated Diabetes, Atherosclerosis and Fatty Liver)
Browse Figures
Review Reports Versions Notes

Abstract

Background: The intestinal ecosystem, including epithelium, immune cells, and microbiota, are influenced by diet and timing of food consumption. The purpose of this study was to evaluate various dietary protocols after ad libitum high fat diet (HFD) consumption on intestinal morphology and mucosal immunity. Methods: C57BL/6 male mice were fed a 45% high fat diet (HFD) for 6 weeks and then randomized to the following protocols; (1) chow, (2) a purified high fiber diet known as the Daniel Fast (DF), HFD consumed (3) ad libitum or in a restricted manner; (4) caloric-restricted, (5) time-restricted (six hours of fasting in each 24 h), or (6) alternate-day fasting (24 h fasting every other day). Intestinal morphology and gut-associated immune parameters were investigated after 2 months on respective protocols. Results: Consuming a HFD resulted in shortening of the intestine and reduction in villi and crypt size. Fasting, while consuming the HFD, did not restore these parameters to the extent seen with the chow and DF diet. Goblet cell number and regulatory T cells had improved recovery with high fiber diets, not seen with the HFD irrespective of fasting. Conclusion: Nutritional content is a critical determinant of intestinal parameters associated with gut health.
Keywords: time-restricted feeding; alternate day fasting; high fat diet (HFD); plant based diet; mucosal immune system; intestinal morphology/histology time-restricted feeding; alternate day fasting; high fat diet (HFD); plant based diet; mucosal immune system; intestinal morphology/histology

Share and Cite

MDPI and ACS Style

Ageeli, R.Y.; Sharma, S.; Puppa, M.; Bloomer, R.J.; Buddington, R.K.; van der Merwe, M. Fasting Protocols Do Not Improve Intestinal Architecture and Immune Parameters in C57BL/6 Male Mice Fed a High Fat Diet. Medicines 2023, 10, 18. https://doi.org/10.3390/medicines10020018

AMA Style

Ageeli RY, Sharma S, Puppa M, Bloomer RJ, Buddington RK, van der Merwe M. Fasting Protocols Do Not Improve Intestinal Architecture and Immune Parameters in C57BL/6 Male Mice Fed a High Fat Diet. Medicines. 2023; 10(2):18. https://doi.org/10.3390/medicines10020018

Chicago/Turabian Style

Ageeli, Raed Y., Sunita Sharma, Melissa Puppa, Richard J. Bloomer, Randal K. Buddington, and Marie van der Merwe. 2023. "Fasting Protocols Do Not Improve Intestinal Architecture and Immune Parameters in C57BL/6 Male Mice Fed a High Fat Diet" Medicines 10, no. 2: 18. https://doi.org/10.3390/medicines10020018

APA Style

Ageeli, R. Y., Sharma, S., Puppa, M., Bloomer, R. J., Buddington, R. K., & van der Merwe, M. (2023). Fasting Protocols Do Not Improve Intestinal Architecture and Immune Parameters in C57BL/6 Male Mice Fed a High Fat Diet. Medicines, 10(2), 18. https://doi.org/10.3390/medicines10020018

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop