Simulated Wildfire Smoke Significantly Alters Sperm DNA Methylation Patterns in a Murine Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.1.1. Ethics Statement
2.1.2. Experimental Design
2.1.3. Douglas Fir Smoke Exposure
2.2. Molecular Analyses
2.2.1. Sperm Collection and DNA Extraction
2.2.2. RRBS Library Construction
2.2.3. Bioinformatics Analyses
2.2.4. Gene Ontology
3. Results
3.1. Overview of DNA Methylation Characteristics
3.2. Simulated Wildfire Smoke-Induced Changes in Sperm DNA Methylation
3.3. GO Term Analysis for DMR-Annotated Genes
3.4. GO Term Analysis for DMRs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schuller, A.; Bellini, C.; Jenkins, T.G.; Eden, M.; Matz, J.; Oakes, J.; Montrose, L. Simulated Wildfire Smoke Significantly Alters Sperm DNA Methylation Patterns in a Murine Model. Toxics 2021, 9, 199. https://doi.org/10.3390/toxics9090199
Schuller A, Bellini C, Jenkins TG, Eden M, Matz J, Oakes J, Montrose L. Simulated Wildfire Smoke Significantly Alters Sperm DNA Methylation Patterns in a Murine Model. Toxics. 2021; 9(9):199. https://doi.org/10.3390/toxics9090199
Chicago/Turabian StyleSchuller, Adam, Chiara Bellini, Timothy G. Jenkins, Matthew Eden, Jacqueline Matz, Jessica Oakes, and Luke Montrose. 2021. "Simulated Wildfire Smoke Significantly Alters Sperm DNA Methylation Patterns in a Murine Model" Toxics 9, no. 9: 199. https://doi.org/10.3390/toxics9090199
APA StyleSchuller, A., Bellini, C., Jenkins, T. G., Eden, M., Matz, J., Oakes, J., & Montrose, L. (2021). Simulated Wildfire Smoke Significantly Alters Sperm DNA Methylation Patterns in a Murine Model. Toxics, 9(9), 199. https://doi.org/10.3390/toxics9090199