Chironomus riparius Proteome Responses to Spinosad Exposure
Abstract
:1. Introduction
2. Material and Methods
2.1. Test Chemicals
2.2. Organism Culture and Exposure
2.3. Protein Extraction
2.4. Sample Preparation for iTRAQ®
2.5. Two-Dimensional Reversed Phase Liquid Chromatography
2.6. Mass Spectrometric Analysis, Protein Identification, and Quantification
2.7. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Treatment | Labeling Reagent | |
---|---|---|
iTRAQ 1 | iTRAQ 2 | |
Control | 121 | 116 |
Control | 115 | 119 |
T1 | 116 | 121 |
T1 | 119 | 118 |
T2 | 114 | 115 |
T2 | 118 | 117 |
T3 | 117 | 113 |
T3 | 113 | 114 |
Total Score | % Cov. | GenBank Accession # | Peptides (95%) | Blast Top Result/Protein Match | Species | Protein Accession # | Significant Changes * |
---|---|---|---|---|---|---|---|
20.00 | 59.0 | KA181893 | 14 | globin VIIA.1 | Chironomus thummi thummi | AAB58930.1 | T2 and T3 |
18.29 | 47.3 | KA184259 | 18 | actin, partial | Zygaena filipendulae | AHW40461.1 | T2 and T3 |
18.23 | 89.4 | KA177778 | 12 | hemoglobin C precursor | Chironomus thummi | AAA28251.1 | T1 |
16.14 | 78.9 | KA193165 | 11 | Globin CTT-VIIB-5/CTT-VIIB-9 | Chironomus thummi thummi | P84298.1 | T1 |
14.00 | 72.9 | KA196492 | 8 | Globin CTT-VIIA; Flags: Precursor | Chironomus thummi thummi | P02226.2 | T3 |
12.00 | 82.0 | KA195409 | 12 | predicted: larval cuticle protein 8-like | Drosophila kikkawai | XP_017017873.1 | T3 |
8.00 | 24.0 | KA178027 | 5 | arginine kinase isoform X3 | Bactrocera dorsalis | XP_029408871.1 | T2 |
4.00 | 14.5 | KA183802 | 3 | elongation factor 1-alpha | Polypedilum vanderplanki | BAH28891 | T2 and T3 |
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Monteiro, H.R.; Pestana, J.L.T.; Soares, A.M.V.M.; Devreese, B.; Lemos, M.F.L. Chironomus riparius Proteome Responses to Spinosad Exposure. Toxics 2020, 8, 117. https://doi.org/10.3390/toxics8040117
Monteiro HR, Pestana JLT, Soares AMVM, Devreese B, Lemos MFL. Chironomus riparius Proteome Responses to Spinosad Exposure. Toxics. 2020; 8(4):117. https://doi.org/10.3390/toxics8040117
Chicago/Turabian StyleMonteiro, Hugo R., João L. T. Pestana, Amadeu M. V. M. Soares, Bart Devreese, and Marco F. L. Lemos. 2020. "Chironomus riparius Proteome Responses to Spinosad Exposure" Toxics 8, no. 4: 117. https://doi.org/10.3390/toxics8040117