Effects of MP Polyethylene Microparticles on Microbiome and Inflammatory Response of Larval Zebrafish
Abstract
:1. Introduction
2. Methods
2.1. Caretaking
2.2. Respirometry
2.3. RNA Extraction, Reverse Transcription (RT), and qPCR
2.4. qPCR
2.5. Respirometry and Gene Expression Statistical Analysis
2.6. Microbiome Analysis
2.6.1. Sampling, DNA Extraction, Sequencing, and Pre-Processing
2.6.2. CLC Analysis
3. Results
3.1. Respirometry
3.2. Gene Expression Analysis
3.3. Structure of Gut Bacteria Communities
3.4. Core Microbial Composition
3.5. Microbial Community Changes through Ontogeny
3.6. MP Exposure Disturbs the Microbiome
4. Discussion
Microbiome
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primer | Reverse Primer | Accession Number |
ef1α51 | CTTCTCAGGCTGACTGTGC | CCGCTAGCATTACCCTCC | AY422992 |
Rpl-13a52 | TCTGGAGGACTGTAAGAGGTATGC | AGACGCACAATCTTGAGAGCAG | NM_212784.1 |
MyD8853 | GAGGCGATTCCAGTAACAGC | GAAAGCATCAAAGGTCTCAGGTG | NM_212814.2 |
Ccl2054 | ATCAATCTGCGCTAATCCATCAC | TGGTGAACATGCTCATCGTCTT | NM_001113595.1 |
L-FABP55 | ACGTGGCAGGTTTACGCTCAG | TTGGAGGTGATGGTGAAGTCG | BC164928.1 |
SOD156 | GTCGTCTGGCTTGTGGAGTG | TGTCAGCGGGCTAGTGCTT | AY324390.1 |
NF-κβ56 | CCAAATCCCAAAAGGTTAGAGATTT | CCTCTTAGGGCTGAGCGAATT | XM_005156814.2 |
Cxcl8a57 | TGTTTTCCTGGCATTTCTGAC | TTTACAGTGTGGGCTTGGAGGG | XM_009306855.3 |
Mus2.150 | TGGTGGACCAGTGTGAAAAA | GGTCCAAAACCCAGCTACAA | XM_021470771.1 |
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Kurchaba, N.; Cassone, B.J.; Northam, C.; Ardelli, B.F.; LeMoine, C.M.R. Effects of MP Polyethylene Microparticles on Microbiome and Inflammatory Response of Larval Zebrafish. Toxics 2020, 8, 55. https://doi.org/10.3390/toxics8030055
Kurchaba N, Cassone BJ, Northam C, Ardelli BF, LeMoine CMR. Effects of MP Polyethylene Microparticles on Microbiome and Inflammatory Response of Larval Zebrafish. Toxics. 2020; 8(3):55. https://doi.org/10.3390/toxics8030055
Chicago/Turabian StyleKurchaba, Nicholas, Bryan J. Cassone, Caleb Northam, Bernadette F. Ardelli, and Christophe M. R. LeMoine. 2020. "Effects of MP Polyethylene Microparticles on Microbiome and Inflammatory Response of Larval Zebrafish" Toxics 8, no. 3: 55. https://doi.org/10.3390/toxics8030055