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Article

Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro

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Laboratorio de Biología Celular y Tisular, Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretaría de la Defensa Nacional, Cerrada de Palomas S/N, Lomas de San Isidro, Alcaldía Miguel Hidalgo, Ciudad de Mexico 11200, Mexico
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Laboratorio de Neurobiología Molecular y Celular, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, Av. Insurgentes Sur # 3877, La Fama, Alcaldía Tlalpan, Ciudad de Mexico 14269, Mexico
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Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de Mexico, Avenida de los Barrios # 1, Los Reyes Iztacala, Tlalnepantla, Estado de Mexico 54090, Mexico
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Laboratorio de Embriología, Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretaría de la Defensa Nacional, Cerrada de Palomas S/N, Lomas de San Isidro, Alcaldía Miguel Hidalgo, Ciudad de Mexico 11200, Mexico
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Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de Mexico, Ciudad Universitaria, Alcaldía Coyoacán, Ciudad de Mexico 04510, Mexico
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Author to whom correspondence should be addressed.
Toxics 2020, 8(3), 51; https://doi.org/10.3390/toxics8030051
Received: 30 June 2020 / Revised: 24 July 2020 / Accepted: 25 July 2020 / Published: 29 July 2020
(This article belongs to the Collection Environmental and Health Risks of Nanotechnology)
Some studies have shown that silicon dioxide nanoparticles (SiO2-NPs) can reach different regions of the brain and cause toxicity; however, the consequences of SiO2-NPs exposure on the diverse brain cell lineages is limited. We aimed to investigate the neurotoxic effects of SiO2-NP (0–100 µg/mL) on rat astrocyte-rich cultures or neuron-rich cultures using scanning electron microscopy, Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR), FTIR microspectroscopy mapping (IQ mapping), and cell viability tests. SiO2-NPs were amorphous particles and aggregated in saline and culture media. Both astrocytes and neurons treated with SiO2-NPs showed alterations in cell morphology and changes in the IR spectral regions corresponding to nucleic acids, proteins, and lipids. The analysis by the second derivative revealed a significant decrease in the signal of the amide I (α-helix, parallel β-strand, and random coil) at the concentration of 10 µg/mL in astrocytes but not in neurons. IQ mapping confirmed changes in nucleic acids, proteins, and lipids in astrocytes; cell death was higher in astrocytes than in neurons (10–100 µg/mL). We conclude that astrocytes were more vulnerable than neurons to SiO2-NPs toxicity. Therefore, the evaluation of human exposure to SiO2-NPs and possible neurotoxic effects must be followed up. View Full-Text
Keywords: cerebellar granule neurons; nanoparticle exposure; neurotoxic effects; secondary astrocytes; silicon dioxide cerebellar granule neurons; nanoparticle exposure; neurotoxic effects; secondary astrocytes; silicon dioxide
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MDPI and ACS Style

Limón-Pacheco, J.H.; Jiménez-Barrios, N.; Déciga-Alcaraz, A.; Martínez-Cuazitl, A.; Mata-Miranda, M.M.; Vázquez-Zapién, G.J.; Pedraza-Chaverri, J.; Chirino, Y.I.; Orozco-Ibarra, M. Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro. Toxics 2020, 8, 51. https://doi.org/10.3390/toxics8030051

AMA Style

Limón-Pacheco JH, Jiménez-Barrios N, Déciga-Alcaraz A, Martínez-Cuazitl A, Mata-Miranda MM, Vázquez-Zapién GJ, Pedraza-Chaverri J, Chirino YI, Orozco-Ibarra M. Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro. Toxics. 2020; 8(3):51. https://doi.org/10.3390/toxics8030051

Chicago/Turabian Style

Limón-Pacheco, Jorge H., Natalie Jiménez-Barrios, Alejandro Déciga-Alcaraz, Adriana Martínez-Cuazitl, Mónica M. Mata-Miranda, Gustavo J. Vázquez-Zapién, Jose Pedraza-Chaverri, Yolanda I. Chirino, and Marisol Orozco-Ibarra. 2020. "Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro" Toxics 8, no. 3: 51. https://doi.org/10.3390/toxics8030051

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