Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice
Abstract
:1. Introduction
2. Experimental Section
2.1. Animals and Handling
2.2. Open Field Locomotor Activity
2.3. Rotarod
2.4. Novel Object Recognition
2.5. Morris Water Maze
Schedule | Platform Size | Platform Location | Cues |
---|---|---|---|
Week 1-Cued | 10 cm | Varies each day | Proximal cue on platform |
Week 2-Acquistion | 10 cm | Southwest | Distal cues on walls |
Week 3-Reversal | 7 cm | Northeast | Distal cues on walls |
Week 4-Shift-reduced | 5 cm | Northwest | Distal cues on walls |
2.6. Fear Conditioning
2.7. Statistical Analysis
3. Results
3.1. Open Field Locomotor Activity
3.2. Rotarod
3.3. Novel Object Recognition
3.4. Morris Water Maze
3.5. Fear Conditioning
Behavior Test | Function | Males | Females |
---|---|---|---|
Open field locomotor | Baseline activity | Higher activity in B6J | Slower habituation in B6N |
Rotarod | Balance and coordination | Greater improvement in B6N over five test days | Significantly longer latencies in B6N; greater improvement in B6N over five tests days |
Novel object recognition | Non-spatial learning and memory | No significant differences | No significant differences |
Morris water maze | Spatial learning and memory | Shorter latencies to escape in B6J; faster swim speeds in B6J; shorter path lengths in shift-reduced in B6N | Shorter path lengths in reversal and shift-reduced in B6N; faster swim speeds in B6J |
Fear conditioning | Fear learning | More freezing in cued phase before tone by B6N | Not tested |
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ashworth, A.; Bardgett, M.E.; Fowler, J.; Garber, H.; Griffith, M.; Curran, C.P. Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice. Toxics 2015, 3, 1-17. https://doi.org/10.3390/toxics3010001
Ashworth A, Bardgett ME, Fowler J, Garber H, Griffith M, Curran CP. Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice. Toxics. 2015; 3(1):1-17. https://doi.org/10.3390/toxics3010001
Chicago/Turabian StyleAshworth, Amy, Mark E. Bardgett, Jocelyn Fowler, Helen Garber, Molly Griffith, and Christine Perdan Curran. 2015. "Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice" Toxics 3, no. 1: 1-17. https://doi.org/10.3390/toxics3010001