Next Article in Journal / Special Issue
Zebrafish as a Model for Developmental Neurotoxicity Assessment: The Application of the Zebrafish in Defining the Effects of Arsenic, Methylmercury, or Lead on Early Neurodevelopment
Previous Article in Journal
Cd, Pb and Hg Biomonitoring in Fish of the Mediterranean Region and Risk Estimations on Fish Consumption
Previous Article in Special Issue
Delayed Behavioral Effects of Early Life Toxicant Exposures in Aquatic Biota
Article

Changes in miRNA Expression Profiling during Neuronal Differentiation and Methyl Mercury-Induced Toxicity in Human in Vitro Models

1
Institute for Health and Consumer Protection, European Commission Joint Research Centre, Ispra (VA) 21027, Italy
2
Department of Biology, University of Konstanz, Konstanz 78464, Germany
3
Department of Experimental and Clinical Medicine, University of Insubria, Varese 21100, Italy
*
Author to whom correspondence should be addressed.
Toxics 2014, 2(3), 443-463; https://doi.org/10.3390/toxics2030443
Received: 4 June 2014 / Revised: 31 July 2014 / Accepted: 5 August 2014 / Published: 29 August 2014
(This article belongs to the Special Issue Developmental Neurotoxicology)
MicroRNAs (miRNAs) are implicated in the epigenetic regulation of several brain developmental processes, such as neurogenesis, neuronal differentiation, neurite outgrowth, and synaptic plasticity. The main aim of this study was to evaluate whether miRNA expression profiling could be a useful approach to detect in vitro developmental neurotoxicity. For this purpose, we assessed the changes in miRNA expression caused by methyl mercury chloride (MeHgCl), a well-known developmental neurotoxicant, comparing carcinoma pluripotent stem cells (NT-2) with human embryonic stem cells (H9), both analyzed during the early stage of neural progenitor commitment into neuronal lineage. The data indicate the activation of two distinct miRNA signatures, one activated upon neuronal differentiation and another upon MeHgCl-induced toxicity. Particularly, exposure to MeHgCl elicited, in both neural models, the down-regulation of the same six out of the ten most up-regulated neuronal pathways, as shown by the up-regulation of the corresponding miRNAs and further assessment of gene ontology (GO) term and pathway enrichment analysis. Importantly, some of these common miRNA-targeted pathways defined in both cell lines are known to play a role in critical developmental processes, specific for neuronal differentiation, such as axon guidance and neurotrophin-regulated signaling. The obtained results indicate that miRNAs expression profiling could be a promising tool to assess developmental neurotoxicity pathway perturbation, contributing towards improved predictive human toxicity testing. View Full-Text
Keywords: miRNAs expression profiling; NT-2 and H9 cell lines; neuronal cultures; pathways of toxicity miRNAs expression profiling; NT-2 and H9 cell lines; neuronal cultures; pathways of toxicity
Show Figures

Figure 1

MDPI and ACS Style

Pallocca, G.; Fabbri, M.; Nerini-Molteni, S.; Pistollato, F.; Zagoura, D.; Sacco, M.G.; Gribaldo, L.; Bremer-Hoffmann, S.; Bal-Price, A. Changes in miRNA Expression Profiling during Neuronal Differentiation and Methyl Mercury-Induced Toxicity in Human in Vitro Models. Toxics 2014, 2, 443-463. https://doi.org/10.3390/toxics2030443

AMA Style

Pallocca G, Fabbri M, Nerini-Molteni S, Pistollato F, Zagoura D, Sacco MG, Gribaldo L, Bremer-Hoffmann S, Bal-Price A. Changes in miRNA Expression Profiling during Neuronal Differentiation and Methyl Mercury-Induced Toxicity in Human in Vitro Models. Toxics. 2014; 2(3):443-463. https://doi.org/10.3390/toxics2030443

Chicago/Turabian Style

Pallocca, Giorgia, Marco Fabbri, Silvia Nerini-Molteni, Francesca Pistollato, Dimitra Zagoura, Maria G. Sacco, Laura Gribaldo, Susanne Bremer-Hoffmann, and Anna Bal-Price. 2014. "Changes in miRNA Expression Profiling during Neuronal Differentiation and Methyl Mercury-Induced Toxicity in Human in Vitro Models" Toxics 2, no. 3: 443-463. https://doi.org/10.3390/toxics2030443

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop