Neonatal AVPR1a Methylation and In-Utero Exposure to Maternal Smoking
Abstract
:1. Introduction
2. Methods
2.1. DNA Methylation
2.2. Array Processing and Quality Control
2.3. Statistical Analysis
3. Results
Individual CpG Sites DNAm
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Non-Smoker (N = 42) | Smoker (N = 10) | p Value | |
---|---|---|---|
N (%) | N (%) | ||
Age | 0.3 | ||
18–20 | 20 (47.6) | 2 (20.0) | |
21–24 | 17 (40.5) | 6 (60.0) | |
25–34 | 5 (11.9) | 2 (20.0) | |
Substance Use | 0.04 | ||
No | 40 (95.2) | 7 (70.0) | |
Yes | 2 (4.8) | 3 (30.0) | |
Race | |||
Non-White | 30 (71.4) | 5 (50.0) | 0.26 |
White | 12 (28.6) | 5 (50.0) | |
Low Income | |||
No | 1 (2.6) | 0 (0.0) | 1 |
Yes | 38 (97.4) | 10 (100.0) | |
Education | 0.5 | ||
Bachelor’s Degree | 1(2.4) | 0 (0) | |
GED | 2 (4.8) | 2 (20.0) | |
High School Diploma | 27 (64.3) | 6 (60.0) | |
Some College or 2 yr. degree | 8 (19.1) | 1 (10.0) | |
Some High School | 2 (4.8) | 1 (10.0) | |
Technical or Trade School | 2 (4.8) | 0 (0.0) | |
Breastfed | 0.05 | ||
No | 3 (7.3) | 3 (37.5) | |
Yes | 38 (92.7) | 5 (62.5) | |
Infant Sex | |||
Female | 22 (52.4) | 6 (60.0) | 0.74 |
Male | 20 (47.6) | 4 (40.0) | |
Preterm Birth | 0.02 | ||
No | 39 (92.9) | 6 (60.0) | |
Yes | 3 (7.1) | 4 (40.0) | |
Medicaid | 1 | ||
No | 2 (5.0) | 0 (0.0) | |
Yes | 38 (95.0) | 10 (100.0) |
Maternal Smoking DNAm by CpG Site | Adjusted Model Coefficient * (95% CI) | p Value ** | Bonferroni | FDR | Location | CPG | Enhancer |
---|---|---|---|---|---|---|---|
cpg12807275 | −0.12 (−0.19, −0.05) | 0.00 | 0.01 | 0.01 | Body | N_Shore | No |
cpg04827692 | −0.04 (−0.06, −0.01) | 0.01 | 0.03 | 0.01 | 1stExon | Island | No |
cpg16668728 | −0.00 (−0.01, 0.01) | 0.72 | 1.00 | 0.81 | 1stExon | Island | Yes |
cpg16352140 | −0.00 (−0.01, 0.01) | 0.40 | 1.00 | 0.53 | 1stExon | Island | Yes |
cpg24501701 | −0.00 (−0.02, 0.01) | 0.81 | 1.00 | 0.81 | 1stExon | Island | Yes |
cpg26727693 | −0.01 (−0.02, 0.01) | 0.29 | 1.00 | 0.47 | 1stExon | Island | Yes |
cpg09208611 | −0.01 (−0.02, −0.00) | 0.00 | 0.03 | 0.01 | 1stExon | Island | Yes |
cpg10906284 | −0.00 (−0.01, 0.00) | 0.13 | 0.65 | 0.26 | 1stExon | Island | Yes |
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Nidey, N.; Bowers, K.; Ding, L.; Ji, H.; Ammerman, R.T.; Yolton, K.; Mahabee-Gittens, E.M.; Folger, A.T. Neonatal AVPR1a Methylation and In-Utero Exposure to Maternal Smoking. Toxics 2023, 11, 855. https://doi.org/10.3390/toxics11100855
Nidey N, Bowers K, Ding L, Ji H, Ammerman RT, Yolton K, Mahabee-Gittens EM, Folger AT. Neonatal AVPR1a Methylation and In-Utero Exposure to Maternal Smoking. Toxics. 2023; 11(10):855. https://doi.org/10.3390/toxics11100855
Chicago/Turabian StyleNidey, Nichole, Katherine Bowers, Lili Ding, Hong Ji, Robert T. Ammerman, Kimberly Yolton, E. Melinda Mahabee-Gittens, and Alonzo T. Folger. 2023. "Neonatal AVPR1a Methylation and In-Utero Exposure to Maternal Smoking" Toxics 11, no. 10: 855. https://doi.org/10.3390/toxics11100855