Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Soybean Sample Extraction
2.3. Immunochromatography
2.4. Electrophoresis and Immunoblotting
2.5. Antibodies Against the Major Soybean Allergens
2.6. ELISA Using Allergen-Specific Antibodies
2.7. Detection of IgE-Binding Proteins using Patient Sera
2.8. Statistical Analysis
3. Results
3.1. Confirmation of Genetically Modified and Non-Genetically Modified Soybeans
3.2. Comparative Levels of Pollinosis-Related Soybean Allergens (Gly m 4 and Gly m 3) in GM-Soybean and Non-GM Soybean Extracts
3.3. Comparative Levels of Other Soybean Allergens in GM-Soybean and non-GM Soybean
3.4. IgE-ELISA and IgE-Immunoblotting using Patient Serum
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Allergen Name | Molecular Mass (kDa) | Features | References |
---|---|---|---|
Soybean food allergens (class 1 food allergens) | |||
Gly m 5 (7S globulin) | 72, 68, 50 | Major storage protein, glycoprotein | [9,10] |
Gly m 6 (11S globulin) | 34, 20 | Major storage protein | [11] |
Gly m 7 | 76 | Seed-specific biotinylated protein (SBP) | [12] |
Gly m Bd 30K (P34) | 34 (30) | Homologous to papain | [13,14] |
Kunitz-type trypsin inhibitor | 18 | trypsin inhibitor | [15] |
Oleosin | 22–24 | Oil body associated protein | [16] |
Pollen-related soybean allergens (class 2 food allergens) | |||
Gly m 3 | 14 | Profilin, Bet v 2 homolog | [17,18] |
Gly m 4 | 17 | PR-10 family, Bet v 1 homolog | [18,19,20,21] |
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Matsuo, A.; Matsushita, K.; Fukuzumi, A.; Tokumasu, N.; Yano, E.; Zaima, N.; Moriyama, T. Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans. Foods 2020, 9, 522. https://doi.org/10.3390/foods9040522
Matsuo A, Matsushita K, Fukuzumi A, Tokumasu N, Yano E, Zaima N, Moriyama T. Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans. Foods. 2020; 9(4):522. https://doi.org/10.3390/foods9040522
Chicago/Turabian StyleMatsuo, Ayato, Kaho Matsushita, Ayano Fukuzumi, Naoki Tokumasu, Erika Yano, Nobuhiro Zaima, and Tatsuya Moriyama. 2020. "Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans" Foods 9, no. 4: 522. https://doi.org/10.3390/foods9040522