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Open AccessCommunication

HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms

1
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany
2
Departamento de Botanica, Facultad de Ciencias Naturales y Oceanograficas, Universidad de Concepcion, Casilla 160-C, Concepcion, Chile
*
Author to whom correspondence should be addressed.
Foods 2020, 9(2), 156; https://doi.org/10.3390/foods9020156
Received: 20 January 2020 / Revised: 29 January 2020 / Accepted: 30 January 2020 / Published: 6 February 2020
High-performance thin-layer chromatography (HPTLC) coupled with negative ion desorption electrospray ionization high-resolution mass spectrometry (DESI-HRMS) was used for the analysis of anthraquinones in complex crude extracts of Chilean dermocyboid Cortinarii. For this proof-of-concept study, the known anthraquinones emodin, physcion, endocrocin, dermolutein, hypericin, and skyrin were identified by their elemental composition. HRMS also allowed the differentiation of the investigated anthraquinones from accompanying compounds with the same nominal mass in the crude extracts. An investigation of the characteristic fragmentation pattern of skyrin in comparison with a reference compound showed, exemplarily, the feasibility of the method for the determination of these coloring, bioactive and chemotaxonomically important marker compounds. Accordingly, we demonstrate that the coupling of HPTLC with DESI-HRMS represents an advanced and efficient technique for the detection of anthraquinones in complex matrices. This analytical approach may be applied in the field of anthraquinone-containing food and plants such as Rheum spp. (rhubarb), Aloe spp., Morinda spp., Cassia spp. and others. Furthermore, the described method can be suitable for the analysis of anthraquinone-based colorants and dyes, which are used in the food, cosmetic, and pharmaceutical industry. View Full-Text
Keywords: HPTLC-MS coupling; HPTLC; negative ion DESI-HR-MS/MS; anthraquinones; Chilean mushrooms; genus Cortinarius HPTLC-MS coupling; HPTLC; negative ion DESI-HR-MS/MS; anthraquinones; Chilean mushrooms; genus Cortinarius
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Laub, A.; Sendatzki, A.-K.; Palfner, G.; Wessjohann, L.A.; Schmidt, J.; Arnold, N. HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms. Foods 2020, 9, 156.

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