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Article

Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation

1
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium
*
Author to whom correspondence should be addressed.
Foods 2020, 9(10), 1511; https://doi.org/10.3390/foods9101511
Received: 21 July 2020 / Revised: 13 October 2020 / Accepted: 20 October 2020 / Published: 21 October 2020
(This article belongs to the Special Issue The Health Benefits of the Bioactive Compounds in Foods)
Quinoa is a kind of nutritious food crop with anti-obesity activity, however, the mechanism is not unclear. In this study, we separated and purified bioactive polysaccharide from quinoa (denoted SQWP-2). The chemical structural was characterized and its effect on 3T3-L1 pre-adipocyte differentiation was evaluated. The molecular weight of SQWP-2 was found to be 7.49 × 103 Da, and the polysaccharide consisted of fructose and glucose. The Glc-(1→, Fru-(2→, →4)-Glcp-(1→, and →4,6)-Glcp-(1→ glycosidic linkages were identified in SQWP-2 through gas chromatography-mass spectrometry. Nuclear magnetic resonance confirmed the monosaccharide composition and glycosidic linkage content, and a suggestion of the structural formula is provided. In Western Blotting and RT-PCR assays, treatment with SQWP-2 significantly inhibited 3T3-L1 differentiation by suppressing PPARγ, C/EBPα, C/EBPβ, C/EBPδ, SREBP1C and AP2 expression. Quinoa polysaccharide isolated here could represent an anti-obesity agent once the structures and differentiation inhibition are definitively characterized. View Full-Text
Keywords: polysaccharide purification; anti-obesity; proliferation; PPARγ polysaccharide purification; anti-obesity; proliferation; PPARγ
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MDPI and ACS Style

Teng, C.; Shi, Z.; Yao, Y.; Ren, G. Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation. Foods 2020, 9, 1511. https://doi.org/10.3390/foods9101511

AMA Style

Teng C, Shi Z, Yao Y, Ren G. Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation. Foods. 2020; 9(10):1511. https://doi.org/10.3390/foods9101511

Chicago/Turabian Style

Teng, Cong, Zhenxing Shi, Yang Yao, and Guixing Ren. 2020. "Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation" Foods 9, no. 10: 1511. https://doi.org/10.3390/foods9101511

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