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Open AccessArticle

A Real-Time PCR Screening Assay for Rapid Detection of Listeria Monocytogenes Outbreak Strains

1
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, via Campo Boario, 64100 Teramo TE, Italy
2
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche Togo Rosati, Via Gaetano Salvemini, 1, 06126 Perugia PG, Italy
3
Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università, 10, 35020 Legnaro PD, Italy
*
Author to whom correspondence should be addressed.
Foods 2020, 9(1), 67; https://doi.org/10.3390/foods9010067
Received: 6 December 2019 / Revised: 2 January 2020 / Accepted: 5 January 2020 / Published: 8 January 2020
(This article belongs to the Special Issue Rapid Methods for Assessing Food Safety and Quality)
From January 2015 to March 2016, an outbreak of 23 human cases of listeriosis in the Marche region and one human case in the Umbria region of Italy was caused by Listeria monocytogenes strains showing a new pulsotype never described before in Italy. A total of 37 clinical strains isolated from patients exhibiting listeriosis symptoms and 1374 strains correlated to the outbreak were received by the Italian National Reference Laboratory for L. monocytogenes (It NRL Lm) of Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) for outbreak investigation. A real-time PCR assay was purposely designed for a rapid screening of the strains related to the outbreak. PCR-positive strains were successively typed through molecular serogrouping, pulsed field gel electrophoresis (PFGE), and Next Generation Sequencing (NGS). Applying the described strategy, based on real-time PCR screening, we were able to considerably reduce time and costs during the outbreak investigation activities. View Full-Text
Keywords: Listeria monocytogenes; outbreak; molecular methods; real-time PCR; screening Listeria monocytogenes; outbreak; molecular methods; real-time PCR; screening
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Torresi, M.; Ruolo, A.; Acciari, V.A.; Ancora, M.; Blasi, G.; Cammà, C.; Centorame, P.; Centorotola, G.; Curini, V.; Guidi, F.; Marcacci, M.; Orsini, M.; Pomilio, F.; Di Domenico, M. A Real-Time PCR Screening Assay for Rapid Detection of Listeria Monocytogenes Outbreak Strains. Foods 2020, 9, 67.

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