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Oligosaccharides Isolated from MGO™ Manuka Honey Inhibit the Adhesion of Pseudomonas aeruginosa, Escherichia Coli O157:H7 and Staphylococcus Aureus to Human HT-29 cells

1
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork P61 C996, Ireland
2
Department of Biological Sciences, Cork Institute of Technology, Cork T12 P928, Ireland
*
Authors to whom correspondence should be addressed.
Foods 2019, 8(10), 446; https://doi.org/10.3390/foods8100446
Received: 17 September 2019 / Revised: 26 September 2019 / Accepted: 27 September 2019 / Published: 1 October 2019
(This article belongs to the Section Nutraceuticals and Functional Foods)
Historically, honey is known for its anti-bacterial and anti-fungal activities and its use for treatment of wound infections. Although this practice has been in place for millennia, little information exists regarding which manuka honey components contribute to the protective nature of this product. Given that sugar accounts for over 80% of honey and up to 25% of this sugar is composed of oligosaccharides, we have investigated the anti-infective activity of manuka honey oligosaccharides against a range of pathogens. Initially, oligosaccharides were extracted from a commercially-available New Zealand manuka honey—MGO™ Manuka Honey (Manuka Health New Zealand Ltd.)—and characterized by High pH anion exchange chromatography coupled with pulsed amperiometric detection. The adhesion of specific pathogens to the human colonic adenocarcinoma cell line, HT-29, was then assessed in the presence and absence of these oligosaccharides. Manuka honey oligosaccharides significantly reduced the adhesion of Escherichia coli O157:H7 (by 40%), Staphylococcus aureus (by 30%), and Pseudomonas aeruginosa (by 52%) to HT-29 cells. This activity was then proven to be concentration dependent and independent of bacterial killing. This study identifies MGO™ Manuka Honey as a source of anti-infective oligosaccharides for applications in functional foods aimed at lowering the incidence of infectious diseases. View Full-Text
Keywords: Manuka honey; oligosaccharides; anti-adhesion; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus Manuka honey; oligosaccharides; anti-adhesion; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus
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Lane, J.A.; Calonne, J.; Slattery, H.; Hickey, R.M. Oligosaccharides Isolated from MGO™ Manuka Honey Inhibit the Adhesion of Pseudomonas aeruginosa, Escherichia Coli O157:H7 and Staphylococcus Aureus to Human HT-29 cells. Foods 2019, 8, 446.

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