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Foods 2019, 8(1), 18; https://doi.org/10.3390/foods8010018

Determination of Pigments in Virgin and Extra-Virgin Olive Oils: A Comparison between Two Near UV-Vis Spectroscopic Techniques

Dipartimento di Chimica e Chimica Industriale, via Moruzzi 13, 56124 Pisa, Italy
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Received: 12 December 2018 / Revised: 2 January 2019 / Accepted: 2 January 2019 / Published: 7 January 2019
(This article belongs to the Special Issue Olive Oil: Processing, Characterization, and Health Benefits)
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Abstract

The colour of olive oil is due to the presence of natural pigments belonging to the class of carotenoids, chlorophylls, and their derivatives. These substances, other than being responsible for the colour, an important qualitative feature of the oil, have antioxidant and, more generally, nutraceutical properties and their quantification can be related to the product’s quality and authenticity. In this work, we have quantified the total amount of carotenoids and chlorophylls’ derivatives in several virgin and extra-virgin olive oils produced in Italy, by using two different methods that are based on near-ultraviolet-visible absorption spectroscopy. The first method defines two indexes, K670 and K470, related to absorbance values of oil at wavelengths of 670 and 470 nm, respectively. The second method is based on the mathematical deconvolution of the whole absorption spectrum of the oil to obtain the concentrations of four main pigments present in olive oils: β-carotene, lutein, pheophytin A, and pheophytin B. The concentrations of the total carotenoids and total chlorophylls’ derivatives, as obtained by the two spectroscopic methods, are compared and the results are discussed in view of the practical usefulness of spectroscopic techniques for a fast determination of pigments in olive oil. View Full-Text
Keywords: extra-virgin olive oil; EVOO; chlorophylls; carotenoids; pigments; colour; quality; spectroscopy; ultraviolet-visible light; light absorption extra-virgin olive oil; EVOO; chlorophylls; carotenoids; pigments; colour; quality; spectroscopy; ultraviolet-visible light; light absorption
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Borello, E.; Domenici, V. Determination of Pigments in Virgin and Extra-Virgin Olive Oils: A Comparison between Two Near UV-Vis Spectroscopic Techniques. Foods 2019, 8, 18.

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