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Foods 2018, 7(6), 82; https://doi.org/10.3390/foods7060082

Detection of β-Lactams and Chloramphenicol Residues in Raw Milk—Development and Application of an HPLC-DAD Method in Comparison with Microbial Inhibition Assays

Laboratory of Food Hygiene and Technology, Veterinary Research Institute, Hellenic Agricultural Organization-DEMETER, Campus of Thermi, 57001 Thessaloniki, Greece
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Received: 27 April 2018 / Revised: 25 May 2018 / Accepted: 27 May 2018 / Published: 1 June 2018
(This article belongs to the Special Issue Toxicity, Detection and Prevention of Food Contaminants)
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Abstract

The present study was carried out to assess the detection sensitivity of four microbial inhibition assays (MIAs) in comparison with the results obtained by the High Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method for antibiotics of the β-lactam group and chloramphenicol in fortified raw milk samples. MIAs presented fairly good results when detecting β-lactams, whereas none were able to detect chloramphenicol at or above the permissible limits. HPLC analysis revealed high recoveries of examined compounds, whereas all detection limits observed were lower than their respective maximum residue limits (MRL) values. The extraction and clean-up procedure of antibiotics was performed by a modified matrix solid phase dispersion procedure using a mixture of Plexa by Agilent and QuEChERS as a sorbent. The HPLC method developed was validated, determining the accuracy, precision, linearity, decision limit, and detection capability. Both methods were used to monitor raw milk samples of several cows and sheep, obtained from producers in different regions of Greece, for the presence of examined antibiotic residues. Results obtained showed that MIAs could be used effectively and routinely to detect antibiotic residues in several milk types. However, in some cases, spoilage of milk samples revealed that the kits’ sensitivity could be strongly affected, whereas this fact does not affect the effectiveness of HPLC-DAD analysis. View Full-Text
Keywords: antibiotic residues; raw milk; HPLC; MSPD; microbial inhibition assays antibiotic residues; raw milk; HPLC; MSPD; microbial inhibition assays
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Karageorgou, E.; Christoforidou, S.; Ioannidou, M.; Psomas, E.; Samouris, G. Detection of β-Lactams and Chloramphenicol Residues in Raw Milk—Development and Application of an HPLC-DAD Method in Comparison with Microbial Inhibition Assays. Foods 2018, 7, 82.

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