Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Analyses
2.2. Molecular Confirmation of L. monocytogenes Strains
2.3. Pulsed-Field Gel Electrophoresis (PFGE) Typing of L. monocytogenes Strains
3. Results and Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Samples | L. monocytogenes Presence |
---|---|
Fresh beef | Negative |
Fresh pork | Negative |
Frozen beef | Negative |
Fat tissue | Negative |
Swab of mixing machine | Negative |
Salted natural swine casings | Negative |
* Cutter swab | Positive |
* Stuffing machine swab | Positive |
* Working desk swab | Positive |
* Swab of meat defrosting area | Positive |
* Mixture—second batch | Positive |
* Desalted natural casings | Positive |
* Mixture in stuffing machine | Positive |
* Dry sausage—fourth ripening day | Positive |
* Dry sausage—seventh ripening day | Positive |
Parameter | Days of Fermentation | Days of Storage | ||||
---|---|---|---|---|---|---|
0 | 4 | 7 | 14 | 14 | 30 | |
pH | 5.37 | 5.12 | 5.15 | 5.23 | 5.33 | 5.43 |
aw | 0.976 | 0.952 | 0.868 | 0.827 | 0.790 | 0.780 |
Salt (%) | 2.5 | 2.56 | 3.05 | 3.75 | 3.42 | 3.30 |
L. monocytogenes (cfu/g) | 600 | 200 | <100 | <100 | <100 | <100 |
L. monocytogenes (25 g) | Positive | Positive | Positive | Negative | Negative | Negative |
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Pažin, V.; Jankuloski, D.; Kozačinski, L.; Dobranić, V.; Njari, B.; Cvrtila, Ž.; Lorenzo, J.M.; Zdolec, N. Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing. Foods 2018, 7, 198. https://doi.org/10.3390/foods7120198
Pažin V, Jankuloski D, Kozačinski L, Dobranić V, Njari B, Cvrtila Ž, Lorenzo JM, Zdolec N. Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing. Foods. 2018; 7(12):198. https://doi.org/10.3390/foods7120198
Chicago/Turabian StylePažin, Valerij, Dean Jankuloski, Lidija Kozačinski, Vesna Dobranić, Bela Njari, Željka Cvrtila, José Manuel Lorenzo, and Nevijo Zdolec. 2018. "Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing" Foods 7, no. 12: 198. https://doi.org/10.3390/foods7120198
APA StylePažin, V., Jankuloski, D., Kozačinski, L., Dobranić, V., Njari, B., Cvrtila, Ž., Lorenzo, J. M., & Zdolec, N. (2018). Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing. Foods, 7(12), 198. https://doi.org/10.3390/foods7120198