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Foods 2018, 7(12), 198; https://doi.org/10.3390/foods7120198

Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing

1
Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
2
Food Institute, Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius” in Skopje, 1000 Skopje, Macedonia
3
Centro Tecnológico de la Carne de Galicia, Rua Galicia Nº 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain
*
Author to whom correspondence should be addressed.
Received: 17 October 2018 / Revised: 19 November 2018 / Accepted: 3 December 2018 / Published: 4 December 2018
(This article belongs to the Special Issue Microbiological Safety of Foods)
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Abstract

In this study, the presence of Listeria monocytogenes was assessed along the production process of fermented sausages in a small-scale facility. Following the isolation of the pathogen from the final product (ISO 11290-1), retrospective sampling was performed during the production of a new batch of sausages, including raw materials, casings, additives, sausage mixtures, sausages during fermentation, and environmental samples. L. monocytogenes was recovered from the following sampling points: the defrosting room and the cuttering, mixing, stuffing, and fermentation phases. Ten strains were isolated, molecularly confirmed as L. monocytogenes by means of a molecular detection system, and subjected to pulsed-field gel electrophoresis (PFGE) typing. On the basis of an unweighted pair group method with arithmetic mean (UPGMA) dendrogram from Ascl pulsotypes, the strains were indistinguishable (no band difference). The same pulsotypes of strains present in both batches of sausages, as well as in environmental samples, indicated the persistence of L. monocytogenes in the sausage production unit. View Full-Text
Keywords: physicochemical parameters; molecular detection assay; pulsed-field gel electrophoresis; foodborne pathogens physicochemical parameters; molecular detection assay; pulsed-field gel electrophoresis; foodborne pathogens
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Pažin, V.; Jankuloski, D.; Kozačinski, L.; Dobranić, V.; Njari, B.; Cvrtila, Ž.; Lorenzo, J.M.; Zdolec, N. Tracing of Listeria monocytogenes Contamination Routes in Fermented Sausage Production Chain by Pulsed-Field Gel Electrophoresis Typing. Foods 2018, 7, 198.

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