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Comparison of R5 and G12 Antibody-Based ELISA Used for the Determination of the Gluten Content in Official Food Samples

Austrian Agency for Health and Food Safety (AGES), Institute for Food Safety, Vienna 1220, Austria
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Christopher J. Smith
Foods 2015, 4(4), 654-664; https://doi.org/10.3390/foods4040654
Received: 20 August 2015 / Revised: 21 October 2015 / Accepted: 27 October 2015 / Published: 9 November 2015
Celiac Disease (CD) is one of the most common food intolerances. It comes along with serious damage of the mucosa in the small intestine and is caused by the storage proteins—termed “gluten”—of wheat, rye, barley and possibly oats. Sensitive individuals need to stick to a strict gluten-free diet. The gluten level in food products labeled as “gluten-free”, must not exceed 20 mg/kg. It is obvious that effective test methods are needed to accurately determine the gluten concentration in foods. The determination of the presence of gluten in foodstuffs is mainly done by means of an immunochemical method called ELISA (enzyme-linked immunosorbent assay). To check the suitability of a G12 antibody-based gluten detection kit for its use in official control systems a number of routine samples were tested in parallel with two different test kits, as would be done in a routine lab. The determination of the gluten content was performed on samples entering the official laboratory including samples from official control plans, commercially available and private samples to request gluten-free labels. The results obtained with the G12 antibody ELISA assay were comparable to the official R5 method. A validation of the two different methods was not part of this study. View Full-Text
Keywords: ELISA; gluten analysis; G12 antibody; R5 antibody; gluten-free; gluten sensitivity; celiac disease ELISA; gluten analysis; G12 antibody; R5 antibody; gluten-free; gluten sensitivity; celiac disease
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Hochegger, R.; Mayer, W.; Prochaska, M. Comparison of R5 and G12 Antibody-Based ELISA Used for the Determination of the Gluten Content in Official Food Samples. Foods 2015, 4, 654-664.

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