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Volume 14
Issue 21
10.3390/foods14213746
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Article

Glycyrrhiza glabra L. Extracts Prevent LPS-Induced Inflammation in RAW264.7 Cells by Targeting Pro-Inflammatory Cytokines, Mediators and the JAK/STAT Signaling Pathway

by
Maria Rosaria Perri
1,†,‡,
Michele Pellegrino
1,†,
Claudia-Crina Toma
2,
Pierfrancesco Prezioso
1,
Vincenzo Tagliaferri
1,
Mariangela Marrelli
1,
Filomena Conforti
1 and
Giancarlo Statti
1,*
1
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy
2
Pharmacognosy Department, Faculty of Pharmacy, Vasile Goldis Western University of Arad, 310045 Arad, Romania
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
Current affiliation: National Research Council of Italy, Institute for Agriculture and Forestry System in the Mediterranean (CNR-ISAFoM), 87036 Rende, Italy
Foods 2025, 14(21), 3746; https://doi.org/10.3390/foods14213746 (registering DOI)
Submission received: 22 September 2025 / Revised: 17 October 2025 / Accepted: 29 October 2025 / Published: 31 October 2025
(This article belongs to the Section Plant Foods)
Versions Notes

Abstract

Glycyrrhiza glabra L. is a species widely spread all over the world, with a long tradition of use in folk medicine. Here, raw and hydrolyzed extracts obtained from roots collected in different geographical areas belonging to the Mediterranean basin were standardized as regards the amount of three main compounds: glycyrrhizin, the most abundant triterpene saponin of licorice, the 18β-glycyrrhetinic acid and the chalcone isoliquiritigenin. Raw and hydrolyzed extracts, as well as their pure single compounds, were investigated for their potential anti-inflammatory properties. The hydrolyzed extracts significantly reduced the production of pro-inflammatory cytokines such as TNF-α, IL-6, NO mediator in LPS-stimulated RAW 264.7 cells. Moreover, they were able to inhibit JAK2 and STAT3 phosphorylated proteins more than pure single standards tested at the same final concentrations, displaying a strength synergism of action. These findings suggest that G. glabra extracts and, more specifically, the hydrolyzed ones could represent interesting sources of potential anti-inflammatory agents able to inhibit the JAK/STAT signaling pathway.
Keywords: Glychyrriza glabra; inflammation; TNF-α; IL-6; nitric oxide; HPLC; isoliquiritigenin; glycyrrhizin; 18β-glycyrrhetinic acid Glychyrriza glabra; inflammation; TNF-α; IL-6; nitric oxide; HPLC; isoliquiritigenin; glycyrrhizin; 18β-glycyrrhetinic acid

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MDPI and ACS Style

Perri, M.R.; Pellegrino, M.; Toma, C.-C.; Prezioso, P.; Tagliaferri, V.; Marrelli, M.; Conforti, F.; Statti, G. Glycyrrhiza glabra L. Extracts Prevent LPS-Induced Inflammation in RAW264.7 Cells by Targeting Pro-Inflammatory Cytokines, Mediators and the JAK/STAT Signaling Pathway. Foods 2025, 14, 3746. https://doi.org/10.3390/foods14213746

AMA Style

Perri MR, Pellegrino M, Toma C-C, Prezioso P, Tagliaferri V, Marrelli M, Conforti F, Statti G. Glycyrrhiza glabra L. Extracts Prevent LPS-Induced Inflammation in RAW264.7 Cells by Targeting Pro-Inflammatory Cytokines, Mediators and the JAK/STAT Signaling Pathway. Foods. 2025; 14(21):3746. https://doi.org/10.3390/foods14213746

Chicago/Turabian Style

Perri, Maria Rosaria, Michele Pellegrino, Claudia-Crina Toma, Pierfrancesco Prezioso, Vincenzo Tagliaferri, Mariangela Marrelli, Filomena Conforti, and Giancarlo Statti. 2025. "Glycyrrhiza glabra L. Extracts Prevent LPS-Induced Inflammation in RAW264.7 Cells by Targeting Pro-Inflammatory Cytokines, Mediators and the JAK/STAT Signaling Pathway" Foods 14, no. 21: 3746. https://doi.org/10.3390/foods14213746

APA Style

Perri, M. R., Pellegrino, M., Toma, C.-C., Prezioso, P., Tagliaferri, V., Marrelli, M., Conforti, F., & Statti, G. (2025). Glycyrrhiza glabra L. Extracts Prevent LPS-Induced Inflammation in RAW264.7 Cells by Targeting Pro-Inflammatory Cytokines, Mediators and the JAK/STAT Signaling Pathway. Foods, 14(21), 3746. https://doi.org/10.3390/foods14213746

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