Schisandra chinensis Bee Pollen Ameliorates Colitis in Mice by Modulating Gut Microbiota and Regulating Treg/Th17 Balance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of SCPE
2.2. Animal Experiment
2.3. Fecal Microbiota Transplantation (FMT)
2.4. Histological Analysis
2.5. Measurement of Inflammatory Mediators
2.6. RNA Isolation and Quantitative Real-Time PCR
2.7. Flow Cytometry Analysis
2.8. Microbiota Analysis
2.9. Measurement of Short-Chain Fatty Acid
2.10. Statistical Analysis
3. Results
3.1. SCPE Altered Composition of Gut Microbiota
3.2. FMT from SCPE-Administration Mice Ameliorated DSS-Induced Colitis
3.3. FMT from SCPE-Administration Mice Improved the Gut Barrier
3.4. Effects of FMT from SCPE-Administration Mice on Inflammation and Oxidative Stress
3.5. FMT from SCPE-Administration Mice Modulated the Response of Immune Cells
3.6. FMT from SCPE-Administration Mice Improved Gut Microbial Structure and Function
3.7. FMT from SCPE-Administration Mice Promoted the Concentration of SCFAs in the Cecum
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | SCFAs Concentrations (mg/g) | |||
---|---|---|---|---|
AA | PA | BA | VA | |
Control | 1.59 ± 0.12 a | 0.63 ± 0.09 a | 1.35 ± 0.53 ab | 0.12 ± 0.04 |
Model | 0.62 ± 0.30 b | 0.26 ± 0.11 b | 0.37 ± 0.06 c | 0.08 ± 0.05 |
LD | 1.05 ± 0.33 a | 0.27 ± 0.14 b | 1.04 ± 0.58 ab | 0.09 ± 0.01 |
HD | 1.40 ± 0.31 a | 0.37 ± 0.11 ab | 1.29 ± 0.41 ab | 0.10 ± 0.03 |
FMTS | 1.40 ± 0.63 a | 0.42 ± 0.15 ab | 1.65 ± 0.51 a | 0.12 ± 0.04 |
FMTC | 1.00 ± 0.46 a | 0.29 ± 0.08 b | 0.49 ± 0.33 c | 0.08 ± 0.02 |
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Cheng, N.; Wang, X.; Zhou, Y.; Zhao, X.; Chen, M.; Zhao, H.; Cao, W. Schisandra chinensis Bee Pollen Ameliorates Colitis in Mice by Modulating Gut Microbiota and Regulating Treg/Th17 Balance. Foods 2024, 13, 585. https://doi.org/10.3390/foods13040585
Cheng N, Wang X, Zhou Y, Zhao X, Chen M, Zhao H, Cao W. Schisandra chinensis Bee Pollen Ameliorates Colitis in Mice by Modulating Gut Microbiota and Regulating Treg/Th17 Balance. Foods. 2024; 13(4):585. https://doi.org/10.3390/foods13040585
Chicago/Turabian StyleCheng, Ni, Xiaochao Wang, Yaoyao Zhou, Xuanxuan Zhao, Minghao Chen, Haoan Zhao, and Wei Cao. 2024. "Schisandra chinensis Bee Pollen Ameliorates Colitis in Mice by Modulating Gut Microbiota and Regulating Treg/Th17 Balance" Foods 13, no. 4: 585. https://doi.org/10.3390/foods13040585