Occurrence, Molecular Serogroups, Antimicrobial Susceptibility and Identification by MALDI-TOF MS of Listeria monocytogenes Isolated from RTE Meat Products in Southern Poland
Abstract
:1. Introduction
2. Materials and Methods
2.1. Food Samples
2.2. Multiplex PCR
2.3. Determination of Antimicrobial Susceptibility
2.4. MALDI TOF MS Identification
3. Results
3.1. Occurrence and Serogroups of L. monocytogenes Isolates
3.2. Antimicrobial Susceptibility of L. monocytogenes Isolates
3.3. Identification of L. monocytogenes Isolates Using MALDI-TOF MS
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene * | Primer Name | Sequence (5′→3′) | Amplicon Size (bp) |
---|---|---|---|
lmo0737 | lmo0737F | AGGGCTTCAAGGACTTACCC | 691 |
lmo0737R | ACGATTTCTGCTTGCCATTC | ||
lmo1118 | lmo1118F | AGGGGTCTTAAATCCTGGAA | 906 |
lmo1118R | CGGCTTGTTCGGCATACTTA | ||
ORF2819 | ORF2819F | AGCAAAATGCCAAAACTCGT | 471 |
ORF2819R | CATCACTAAAGCCTCCCATTG | ||
ORF2110 | ORF2110F | AGTGGACAATTGATTGGTGAA | 597 |
ORF2110R | CATCCATCCCTTACTTTGGAC | ||
prs | prsR | GCTGAAGAGATTGCGAAAGAAG | 307 |
prsF | CAAAGAAACCTTGGATTTGCGG |
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Pyz-Łukasik, R.; Piróg-Komorowska, A.; Policht, A. Occurrence, Molecular Serogroups, Antimicrobial Susceptibility and Identification by MALDI-TOF MS of Listeria monocytogenes Isolated from RTE Meat Products in Southern Poland. Foods 2024, 13, 2950. https://doi.org/10.3390/foods13182950
Pyz-Łukasik R, Piróg-Komorowska A, Policht A. Occurrence, Molecular Serogroups, Antimicrobial Susceptibility and Identification by MALDI-TOF MS of Listeria monocytogenes Isolated from RTE Meat Products in Southern Poland. Foods. 2024; 13(18):2950. https://doi.org/10.3390/foods13182950
Chicago/Turabian StylePyz-Łukasik, Renata, Anna Piróg-Komorowska, and Agata Policht. 2024. "Occurrence, Molecular Serogroups, Antimicrobial Susceptibility and Identification by MALDI-TOF MS of Listeria monocytogenes Isolated from RTE Meat Products in Southern Poland" Foods 13, no. 18: 2950. https://doi.org/10.3390/foods13182950
APA StylePyz-Łukasik, R., Piróg-Komorowska, A., & Policht, A. (2024). Occurrence, Molecular Serogroups, Antimicrobial Susceptibility and Identification by MALDI-TOF MS of Listeria monocytogenes Isolated from RTE Meat Products in Southern Poland. Foods, 13(18), 2950. https://doi.org/10.3390/foods13182950