Pectin Extraction from Residues of the Cocoa Fruit (Theobroma cacao L.) by Different Organic Acids: A Comparative Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Raw Materials
2.2. Chemicals
2.3. Preparation of Dehydrated Cocoa Pod Husk
2.4. Pectin Extraction Experiments from Dehydrated CPH
2.5. Central Composite Design of Experiments Using Response Surface Methodology
2.6. Characterisation of CHP-Pectins
2.6.1. FTIR Analysis of CHP-Pectins
2.6.2. Determination of Equivalent Weight, Methoxyl Content (MeO), Anhydrouronic Acid Content (AUA) and Degree of Esterification (DE) of CHP-Pectins
3. Results
3.1. CCD Experiments and the Model Analysis
3.2. Optimisation of Pectin Yield Model
3.3. Confirmation Experiments for the Pectin Yield Model
3.4. Characterisation of the Pectin from d-CPH Using Three Organic Acids
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Coded Factors | ||||||
---|---|---|---|---|---|---|
−1.414 | −1.000 | 0.000 | +1.000 | +1.414 | ||
A: Time (min) | 60 | 64 | 75 | 86 | 90 | |
B: Temperature (°C) | 70 | 73 | 80 | 87 | 90 | |
C: Organic acid | C [1] citric acid | C [2] malic acid | C [3] fumaric acid |
Std. | Block | Run | A: Time (min) | B: Temperature (°C) | C: Organic-Acid (−) | Yield (g/kg) |
---|---|---|---|---|---|---|
32 | 1 | 1 | 86 | 87 | Fumaric acid | 4.0 |
31 | 1 | 2 | 64 | 87 | Fumaric acid | 3.6 |
17 | 1 | 3 | 64 | 87 | Malic acid | 2.4 |
33 | 1 | 4 | 75 | 80 | Fumaric acid | 1.0 |
18 | 1 | 5 | 86 | 87 | Malic acid | 5.4 |
4 | 1 | 6 | 86 | 87 | Citric acid | 4.6 |
21 | 1 | 7 | 75 | 80 | Malic acid | 4.4 |
29 | 1 | 8 | 64 | 73 | Fumaric acid | 1.6 |
5 | 1 | 9 | 75 | 80 | Citric acid | 5.0 |
1 | 1 | 10 | 64 | 73 | Citric acid | 4.2 |
15 | 1 | 11 | 64 | 73 | Malic acid | 2.2 |
6 | 1 | 12 | 75 | 80 | Citric acid | 5.0 |
19 | 1 | 13 | 75 | 80 | Malic acid | 4.8 |
16 | 1 | 14 | 86 | 73 | Malic acid | 4.6 |
3 | 1 | 15 | 64 | 87 | Citric acid | 1.8 |
35 | 1 | 16 | 75 | 80 | Fumaric acid | 1.0 |
30 | 1 | 17 | 86 | 73 | Fumaric acid | 1.4 |
2 | 1 | 18 | 86 | 73 | Citric acid | 4.2 |
20 | 1 | 19 | 75 | 80 | Malic acid | 4.4 |
34 | 1 | 20 | 75 | 80 | Fumaric acid | 4.6 |
7 | 1 | 21 | 75 | 80 | Citric acid | 5.8 |
23 | 2 | 22 | 90 | 80 | Malic acid | 5.6 |
40 | 2 | 23 | 75 | 80 | Fumaric acid | 1.0 |
28 | 2 | 24 | 75 | 80 | Malic acid | 4.4 |
9 | 2 | 25 | 90 | 80 | Citric acid | 9.4 |
10 | 2 | 26 | 75 | 70 | Citric acid | 6.8 |
22 | 2 | 27 | 60 | 80 | Malic acid | 1.6 |
12 | 2 | 28 | 75 | 80 | Citric acid | 5.0 |
38 | 2 | 29 | 75 | 70 | Fumaric acid | 2.4 |
24 | 2 | 30 | 75 | 70 | Malic acid | 3.6 |
14 | 2 | 31 | 75 | 80 | Citric acid | 4.8 |
41 | 2 | 32 | 75 | 80 | Fumaric acid | 1.2 |
26 | 2 | 33 | 75 | 80 | Malic acid | 4.6 |
11 | 2 | 34 | 75 | 90 | Citric acid | 5.2 |
37 | 2 | 35 | 90 | 80 | Fumaric acid | 4.0 |
8 | 2 | 36 | 60 | 80 | Citric acid | 5.4 |
39 | 2 | 37 | 75 | 90 | Fumaric acid | 3.0 |
13 | 2 | 38 | 75 | 80 | Citric acid | 5.0 |
25 | 2 | 39 | 75 | 90 | Malic acid | 5.0 |
27 | 2 | 40 | 75 | 80 | Malic acid | 4.4 |
42 | 2 | 41 | 75 | 80 | Fumaric acid | 1.0 |
36 | 2 | 42 | 60 | 80 | Fumaric acid | 0.8 |
Source | Sum of Squares | df | Mean Square | F-Value | p-Value | |
---|---|---|---|---|---|---|
Block | 1.60 | 1 | 1.60 | |||
Model | 88.00 | 3 | 29.33 | 20.70 | <0.0001 | significant |
A-Time | 24.48 | 1 | 24.48 | 17.28 | 0.0002 | |
C-OA 1 | 63.52 | 2 | 31.76 | 22.41 | <0.0001 | |
Residual | 52.43 | 37 | 1.42 | |||
Lack of Fit | 43.18 | 25 | 1.73 | 2.24 | 0.0728 | not significant |
Pure Error | 9.25 | 12 | 0.7711 | |||
Cor. Total | 142.03 | 41 |
Factor | Goal | Lower Limit | Upper Limit | Importance |
---|---|---|---|---|
A: Time | In range | 64.39 | 85.61 | 3 |
B: Temperature | Equal to 80.0 | 72.93 | 90.00 | 3 |
C: Organic acid | In range | Citric acid | Fumaric acid | 3 |
Yield | Maximise | 0.80 | 9.40 | 5 |
Experiment | Time | Temperature | Organic Acid | |
---|---|---|---|---|
1 | 85.607 | 80.00 | Citric acid | 6.167 |
2 | 85.607 | 80.00 | Malic acid | 5.110 |
3 | 85.607 | 80.00 | Fumaric acid | 3.196 |
Organic Acid | Predicted Mean | Std. Dev. | n | SE Pred. | 95% PI Low | Data Mean | 95% PI High |
---|---|---|---|---|---|---|---|
Citric acid | 6.17 | 1.19 | 6 | 0.63 | 4.89 | 5.73 | 7.44 |
Malic acid | 5.11 | 1.19 | 6 | 0.63 | 3.83 | 4.7 | 6.39 |
Fumaric acid | 3.2 | 1.19 | 6 | 0.63 | 1.92 | 3.53 | 4.47 |
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Jarrín-Chacón, J.P.; Núñez-Pérez, J.; Espín-Valladares, R.d.C.; Manosalvas-Quiroz, L.A.; Rodríguez-Cabrera, H.M.; Pais-Chanfrau, J.M. Pectin Extraction from Residues of the Cocoa Fruit (Theobroma cacao L.) by Different Organic Acids: A Comparative Study. Foods 2023, 12, 590. https://doi.org/10.3390/foods12030590
Jarrín-Chacón JP, Núñez-Pérez J, Espín-Valladares RdC, Manosalvas-Quiroz LA, Rodríguez-Cabrera HM, Pais-Chanfrau JM. Pectin Extraction from Residues of the Cocoa Fruit (Theobroma cacao L.) by Different Organic Acids: A Comparative Study. Foods. 2023; 12(3):590. https://doi.org/10.3390/foods12030590
Chicago/Turabian StyleJarrín-Chacón, Jenny Paola, Jimmy Núñez-Pérez, Rosario del Carmen Espín-Valladares, Luis Armando Manosalvas-Quiroz, Hortensia María Rodríguez-Cabrera, and José Manuel Pais-Chanfrau. 2023. "Pectin Extraction from Residues of the Cocoa Fruit (Theobroma cacao L.) by Different Organic Acids: A Comparative Study" Foods 12, no. 3: 590. https://doi.org/10.3390/foods12030590