Next Article in Journal
Occurrence of Glyphosate and Other Polar Pesticides in Honey from Lombardy and Emilia-Romagna Regions in Italy: Three-Year Monitoring Results
Previous Article in Journal
Free and Bound Phenolic Profiles and Antioxidant Activities in Melon (Cucumis melo L.) Pulp: Comparative Study on Six Widely Consumed Varieties Planted in Hainan Province
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Foods
Volume 12
Issue 24
10.3390/foods12244447
foods-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Intelligent Electrochemical Point-of-Care Test Method with Interface Control Based on DNA Pyramids: Aflatoxin B1 Detection in Food and the Environment

by
Wenqin Wu
1,
Yizhen Bai
1,
Tiantian Zhao
1,
Meijuan Liang
1,
Xiaofeng Hu
1,
Du Wang
1,
Xiaoqian Tang
1,
Li Yu
1,
Qi Zhang
1,
Peiwu Li
1 and
Zhaowei Zhang
1,2,*
1
Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, National Reference Laboratory for Agricultural Testing (Biotoxin), Hubei Hongshan Lab, Wuhan 430062, China
2
School of Bioengineering and Health, State Key Laboratory of New Textile Materials and Advanced Processing Technologies, Wuhan Textile University, Wuhan 430200, China
*
Author to whom correspondence should be addressed.
Foods 2023, 12(24), 4447; https://doi.org/10.3390/foods12244447
Submission received: 23 November 2023 / Revised: 6 December 2023 / Accepted: 6 December 2023 / Published: 12 December 2023
(This article belongs to the Section Food Quality and Safety)
Browse Figures
Versions Notes

Abstract

Sensitive, intelligent point-of-care test (iPOCT) methods for small molecules like aflatoxin B1 (AFB1) are urgently needed for food and the environment. The challenge remains of surface control in iPOCT. Herein, we developed an electrochemical sensor based on the DNA pyramid (DNP), combining a smartphone, app, and mobile electrochemical workstations to detect AFB1. The DNP’s structure can reduce local overcrowding and entanglement between neighboring probes, control the density and orientation of recognition probes (antibodies), produce uniform and orientational surface assemblies, and improve antigen–antibody-specific recognition and binding efficiency. Simultaneously, the hollow structure of the DNP enhances the electron transfer capacity and increases the sensitivity of electrochemical detection. In this work, the biosensor based on DNP was first combined with electrochemical (Ec) iPOCT to simultaneously achieve ordered interface modulation of recognition probes and intelligent detection of AFB1. Under optimal conditions, we found a detection limit of 3 pg/mL and a linear range of 0.006–30 ng/mL (R2 = 0.995). Further, using peanut, soybean, corn, and lake water as complex matrices, it recorded recoveries of 82.15–100.53%, excellent selectivity, acceptable stability, and good reproducibility. Finally, this Ec iPOCT provides consistent results compared to the high-performance liquid chromatography-tandem mass spectrometry method.
Keywords: intelligent POCT; aflatoxin B1; DNA pyramid; screen-printed gold electrode; electrochemical biosensor intelligent POCT; aflatoxin B1; DNA pyramid; screen-printed gold electrode; electrochemical biosensor

Share and Cite

MDPI and ACS Style

Wu, W.; Bai, Y.; Zhao, T.; Liang, M.; Hu, X.; Wang, D.; Tang, X.; Yu, L.; Zhang, Q.; Li, P.; et al. Intelligent Electrochemical Point-of-Care Test Method with Interface Control Based on DNA Pyramids: Aflatoxin B1 Detection in Food and the Environment. Foods 2023, 12, 4447. https://doi.org/10.3390/foods12244447

AMA Style

Wu W, Bai Y, Zhao T, Liang M, Hu X, Wang D, Tang X, Yu L, Zhang Q, Li P, et al. Intelligent Electrochemical Point-of-Care Test Method with Interface Control Based on DNA Pyramids: Aflatoxin B1 Detection in Food and the Environment. Foods. 2023; 12(24):4447. https://doi.org/10.3390/foods12244447

Chicago/Turabian Style

Wu, Wenqin, Yizhen Bai, Tiantian Zhao, Meijuan Liang, Xiaofeng Hu, Du Wang, Xiaoqian Tang, Li Yu, Qi Zhang, Peiwu Li, and et al. 2023. "Intelligent Electrochemical Point-of-Care Test Method with Interface Control Based on DNA Pyramids: Aflatoxin B1 Detection in Food and the Environment" Foods 12, no. 24: 4447. https://doi.org/10.3390/foods12244447

APA Style

Wu, W., Bai, Y., Zhao, T., Liang, M., Hu, X., Wang, D., Tang, X., Yu, L., Zhang, Q., Li, P., & Zhang, Z. (2023). Intelligent Electrochemical Point-of-Care Test Method with Interface Control Based on DNA Pyramids: Aflatoxin B1 Detection in Food and the Environment. Foods, 12(24), 4447. https://doi.org/10.3390/foods12244447

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop