Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains
2.2. Linalool
2.3. Treatment of LM by Linalool
2.4. iTRAQ-Based Quantitative Proteomics Analysis
2.4.1. Protein Extraction, Digestion, and Labeling with iTRAQ Reagents
2.4.2. Chromatographic Separation and LC-MS/MS Analysis
2.4.3. Proteomic Analysis
2.5. Flow Cytometry Analysis
2.5.1. Membrane Permeability
2.5.2. Membrane Potential
2.5.3. Efflux Activity
2.5.4. Respiratory Activity
2.6. Statistical Analysis
3. Results
3.1. Quality Assessment of Proteomics Sequencing
3.2. Functional Annotation and Analysis of Proteins
3.3. Analysis of DEPs
3.4. The GO Enrichment Analysis
3.5. The KEGG Enrichment Analysis
3.6. Flow Cytometry Analysis
4. Discussion
4.1. Proteomic Technology Used in the Antimicrobial Research Field
4.2. Further Analysis of Important Functions in GO Enrichment Analysis
4.3. Further Analysis of Important Pathways in KEGG Enrichment Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gao, Z.; Zhong, W.; Liu, T.; Zhao, T.; Guo, J. Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool. Foods 2021, 10, 2449. https://doi.org/10.3390/foods10102449
Gao Z, Zhong W, Liu T, Zhao T, Guo J. Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool. Foods. 2021; 10(10):2449. https://doi.org/10.3390/foods10102449
Chicago/Turabian StyleGao, Zhipeng, Weiming Zhong, Ting Liu, Tianyu Zhao, and Jiajing Guo. 2021. "Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool" Foods 10, no. 10: 2449. https://doi.org/10.3390/foods10102449