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Open AccessArticle

Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach

1
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Department of Immunology and Food Microbiology, Tuwima 10, 10-748 Olsztyn, Poland
2
Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria
*
Authors to whom correspondence should be addressed.
Foods 2021, 10(1), 163; https://doi.org/10.3390/foods10010163
Received: 7 December 2020 / Revised: 28 December 2020 / Accepted: 11 January 2021 / Published: 14 January 2021
The main food-origin antigens that the infant’s body is in contact with are cow’s milk proteins (CMP). Still, CMP are one of the main sources of beneficial biologically active peptides that play a role in treatment of non-communicable diseases. Safe methods to quickly predict the sensitizing potential of food proteins among their range of health-promoting properties are essential. The aim of study was to adapt an integrated approach combining several in silico (IS) studies and in vitro (IV) assays to screen the multifunctionality of CMP-derived peptides. Major histocompatability complex type II MHC II-binders, interleukin-4 and -10 inducers, interferon γ -inducers and immunobioactivity tools were used to predict the peptide-power of inducing allergies or tolerance. A comparison of the peptide profiless revealed the presence of one identical and one overlapping sequence in IS and IV hydrolysate. By IS analysis, four of 24 peptides were found to have high affinity and stimulate IL-4 expression, and by IV, one of seven peptides had this potential (Bos d9 peptide DIPNPIGSENSEK (195–208)). Three IV peptides may induce IL-10 expression. The IV/IS assessment seems promising agents for peptides’ potential determination dedicated only to preliminary screening of peptides. The IV verification is still crucial in further steps of studies. View Full-Text
Keywords: allergy; cow’s milk allergens; in silico tools; MHC II binded peptides; IL-4 inducing peptides; IFNγ inducing peptides; biologically-active peptides allergy; cow’s milk allergens; in silico tools; MHC II binded peptides; IL-4 inducing peptides; IFNγ inducing peptides; biologically-active peptides
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MDPI and ACS Style

Ogrodowczyk, A.M.; Dimitrov, I.; Wróblewska, B. Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach. Foods 2021, 10, 163. https://doi.org/10.3390/foods10010163

AMA Style

Ogrodowczyk AM, Dimitrov I, Wróblewska B. Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach. Foods. 2021; 10(1):163. https://doi.org/10.3390/foods10010163

Chicago/Turabian Style

Ogrodowczyk, Anna M.; Dimitrov, Ivan; Wróblewska, Barbara. 2021. "Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach" Foods 10, no. 1: 163. https://doi.org/10.3390/foods10010163

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