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Open AccessArticle

Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation

1
Institute of Oral Medicine, National Cheng Kung University, Tainan 709, Taiwan
2
Department of Stomatology, National Cheng Kung University Hospital, Tainan 709, Taiwan
3
Department of Biomaterial Science, School of Dentitry, University of Michigan, Ann Arbor, MI 48109, USA
4
Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor MI 48109, USA
*
Author to whom correspondence should be addressed.
Dent. J. 2018, 6(4), 70; https://doi.org/10.3390/dj6040070
Received: 26 October 2018 / Revised: 24 November 2018 / Accepted: 5 December 2018 / Published: 10 December 2018
(This article belongs to the Special Issue Restorative and Esthetic Dentistry)
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Abstract

Phosphophoryn (PP) and dentin sialoprotein (DSP) are two of the most abundant dentin matrix non-collagenous proteins, and are derived from dentin sialoprotein-phosphophoryn (DSP-PP) mRNA. Mutations in the DSP-PP gene are linked to dentinogenesis imperfecta II and III. Previously, we reported transient DSP-PP expression in preameloblast cells first, followed by co-expression in preameloblasts and young odontoblasts, and finally sustained expression in odontoblasts. This phenomenon raised the possibility that DSP/PP proteins secreted by preameloblasts might promote dental pulp cell migration toward the dental pulp border and promote dental pulp cell differentiation. To examine the effects of DSP/PP proteins on dental pulp cell development, we investigated:(1) native PP effects on dental pulpcell migration and matrix protein expression; and (2) recombinant DSP/PP protein effects on cell proliferation and differentiation. We found that PP promoted cell migration and the expression of high levels of Col type I and PP in dental pulp cells. The addition of recombinant DSP/PP proteins affected cell proliferation and differentiation in a dental pulp cell line. These findings strongly suggest that DSP/PP may modulate cell migration, cell proliferation and differentiation, thus leading to dentin formation. DSP/PP protein may be useful clinically for pulp tissue regeneration. View Full-Text
Keywords: phosphophoryn (PP); dentin sialoprotein (DSP); dentinogenesis; dental pulp cells; cell migration; differentiation phosphophoryn (PP); dentin sialoprotein (DSP); dentinogenesis; dental pulp cells; cell migration; differentiation
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Chuang, S.-F.; Chen, Y.-H.; Ma, P.; Ritchie, H.H. Phosphophoryn and Dentin Sialoprotein Effects on Dental Pulp Cell Migration, Proliferation, and Differentiation. Dent. J. 2018, 6, 70.

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