Vitamin D in Peri-Implant and Periodontal Tissue
Abstract
1. Introduction
2. Role of Vitamin D in Peri-Implant Tissue
Authors and Year | Study Type | Population (N), Sex and Age | Details of Vitamin D | Conclusion |
---|---|---|---|---|
Wu et al. (2013) [24] | In vivo | Wistar male rats N = 30 Age (weeks) = 10–11 | Vitamin D3 12 μg/kg/day via gavage for 14 days (started 3 days after implant surgery). Co-intervention with insulin subcutaneously twice daily (5.5 IU at 8 p.m., 3.5 IU at 8:00 a.m.). | Both insulin and vitamin D3 alone improved some outcomes such as implant fixation in diabetic rats, but combination therapy had superior effects. |
Gomes-Ferreira et al. (2023) [25] | In vivo | Wistar male rats N = 24 Age not reported | Vitamin D 0.1 µg/kg/day via gavage, for 30 days. Co-intervention with daily subcutaneous teriparatide 0.5 µg/kg/day. | Vitamin D combined with teriparatide in orchiectomized rats with induced osteoporosis significantly increased bone volume and improved bone quality around tibial implants. |
Pitol-Palin et al. (2025) [26] | In vivo | Wistar male rats N = 15 Age (months) = 3 | Dip-coating of titanium implants with vitamin D3 (Addera D3®). Concentrations tested: vD40 µL: 40 µL vitamin D3 in 100 mL DMSO; vD400 µL: 400 µL vitamin D3 in 100 mL DMSO. | The vD400 µL concentration led to improved bone microarchitecture. |
Salomó-Coll et al. (2016) [3] | In vivo | Dogs (American Foxhound) N = 6 Sex not specified Age (years) = 1 | Implant submerged in 10% vitamin D2 (ergocalciferol) 10% solution. | Topical application of vitamin D on immediate implants did not significantly enhance osseointegration compared to controls. However, vitamin-D-treated implants showed less crestal bone loss and about 10% higher BIC contact after 12 weeks. |
Ayyad et al. (2025) [4] | Randomized controlled trial | Healthy ASA I N = 24 Sex not specified Age (years): 21–40 years | Topical vitamin D3 (calcitriol) 1 µg/mL emulgel applied to both the implant surface and osteotomy site immediately before implant placement. | Topical vitamin D3 improved peri-implant soft tissue healing (reduced probing depth and bleeding index), decreased postoperative pain, and enhanced implant stability and bone density over 6 months. |
Dvorak et al. (2012) [27] | In vivo | Ovariectomized female rats N = 48 Age (months) = 3 | Vitamin D was provided via diet containing 1000 IU vitamin D3 per kg of feed. | Dietary vitamin D significantly improved BIC and peri-implant bone density in ovariectomized rats. |
Cheng et al. (2024) [2] | Retrospective case-control | Wistar male rats N = 24 Age (weeks) = 8 | Vitamin D3 supplementation of 2500 IU/kg diet for 6 weeks prior to surgery and continuing until sacrifice. | Vitamin D supplementation improved osseointegration, as evidenced by increased BIC and higher removal torque values compared to the control group. |
Tabrizi et al. (2022) [28] | Prospective cohort | Healthy N = 40 18 males and 22 females Age (years) = 18–50 | Oral vitamin D3 capsules of 1000 IU/day, starting 6 weeks before surgery and continuing for 3 months after implant placement. | Oral vitamin D3 supplementation improved early osseointegration, showing higher implant stability quotient values at 6 and 12 weeks compared to placebo. |
2.1. Peri-Implant Bone Healing
2.2. Peri-Implantitis
3. Role of Vitamin D in Periodontal Disease
4. Overview of Concepts and Findings
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
VD3 | Vitamin D3 (cholecalciferol) |
BIC | Bone-to-Implant Contact |
ITB | Interthread Bone |
NBF | New Bone Formation |
HLM | Histological Linear Measurement |
BV | Bone Volume |
BV/TV | Bone Volume/Tissue Volume |
Tb.Th | Trabecular Thickness |
Tb.N | Trabecular Number |
Tb.Sp | Trabecular Separation |
Po-tot | Total Porosity |
MBL | Marginal Bone Loss |
PDL | Periodontal Ligament |
MMP-9 | Matrix Metalloproteinase-9 |
GCF | Gingival Crevicular Fluid |
PPD | Periodontal Probing Depth |
CAL | Clinical Attachment Level |
BOP | Bleeding on Probing |
BMI | Body Mass Index |
IL | Interleukin (IL-1, IL-6, IL-10) |
TNF-α | Tumor Necrosis Factor Alpha |
RANKL | Receptor Activator of Nuclear Factor Kappa-B Ligand |
OCN | Osteocalcin |
OPN | Osteopontin |
ALP | Alkaline Phosphatase |
RUNX2 | Runt-Related Transcription Factor 2 |
ASPN | Asporin |
BMP-2 | Bone Morphogenetic Protein 2 |
VDR | Vitamin D Receptor |
COPD | Chronic Obstructive Pulmonary Disease |
PI | Plaque Index |
GBI | Gingival Bleeding Index |
SRP | Scaling and Root Planing |
IU | International Units |
UI/kg | International Units per Kilogram |
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Authors and Year | Study Type | Population (N), Sex and Age | Details of Vitamin D | Conclusion |
---|---|---|---|---|
Laky et al. (2017) [30] | Observational case–control | Periodontal disease N = 29 12 males and 17 females Mean age (years) = 35.41 ± 7.7 Healthy N = 29 8 males and 21 females Mean age (years) = 35.45 ± 7.4 | Serum 25(OH)D levels were measured, but no supplementation was given. Vitamin D deficiency was defined as serum levels < 50 nmol/L. | Vitamin D deficiency (<50 nmol/L) was significantly associated with periodontal disease, with affected patients showing lower serum levels than healthy controls. |
Yildirim et al. (2025) [31] | Observational comparative | Generalized stage III–IV periodontitis N = 63 38 females and 25 males Mean age (years) = 46.0 ± 8.0 | Serum 25(OH)D levels were measured using ELISA. Vitamin D deficiency was defined as <20 ng/mL, insufficiency as 20–30 ng/mL, and sufficiency as >30 ng/mL. | Lower serum vitamin D levels were linked to higher MMP-8 activity, suggesting a role in increased periodontal tissue destruction. |
Nebel et al. (2015) [32] | In vitro | Chronic periodontitis N = 12 Gingival tissue samples were collected from males and females Age (years) = 30–65 | PDL cells were treated with 1α,25-dihydroxyvitamin D3 at concentrations of 10−8 M and 10−7 M. | Vitamin D3 stimulated bone-forming activity and suppressed inflammation in periodontal ligament cells. |
Pereira et al. (2024) [33] | Observational cross-sectional | Osteoporosis N = 15 Mean age (years) = 57.3 ± 4.9 Healthy N = 15 Mean age (years) = 57.0 ± 4.1 | Serum 25(OH)D levels were measured using electrochemiluminescence immunoassay. Deficiency was defined as <20 ng/mL, insufficiency as 20–29 ng/mL, and sufficiency as ≥30 ng/mL. | Lower vitamin D levels were associated with more severe periodontal attachment loss in postmenopausal women with osteoporosis. |
Han et al. (2019) [34] | In vivo | Wistar male rats N = 40 Age (weeks) = 8 | 25(OH)D3 via intraperitoneal injection 5 μg/kg, 3 times per week, 12 weeks. | 25(OH)D3 diminished bone loss, lung damage, and systemic inflammation in rats with periodontitis and/or COPD. |
Ramaprabha et al. (2023) [1] | Randomized clinical trial | Non-diabetic male N = 46 Diabetic male N = 46 Age (years) = 35–60 | Oral vitamin D3 granules, 60,000 IU once a week for 8 weeks after scaling and root planing. | Vitamin D supplementation alongside scaling and root planing significantly improved periodontal health and serum vitamin D levels, with greater benefits in patients without diabetes. |
Mahendra et al. (2025) [29] | Retrospective cohort | N (patients) = 143 N (implants) = 161 Male = 54.7% Female = 45.3% Age (years) = 18–62 | Past medical history included self-reported vitamin D deficiency (6.3% of participants). Vitamin D deficiency was assessed from patient records but was not significantly associated with implant survival. | Dental implant survival is high over five years, but success depends on systemic health, habits, and prosthetic factors, while vitamin D deficiency alone is not a predictor of failure. |
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Duarte, F.d.S.; Duarte, N.D.; Mulinari-Santos, G.; Frigério, P.B.; Okamoto, R.; Buchaim, R.L.; Buchaim, D.V.; Issa, J.P.M. Vitamin D in Peri-Implant and Periodontal Tissue. Dent. J. 2025, 13, 448. https://doi.org/10.3390/dj13100448
Duarte FdS, Duarte ND, Mulinari-Santos G, Frigério PB, Okamoto R, Buchaim RL, Buchaim DV, Issa JPM. Vitamin D in Peri-Implant and Periodontal Tissue. Dentistry Journal. 2025; 13(10):448. https://doi.org/10.3390/dj13100448
Chicago/Turabian StyleDuarte, Felipe de Souza, Nathália Dantas Duarte, Gabriel Mulinari-Santos, Paula Buzo Frigério, Roberta Okamoto, Rogerio Leone Buchaim, Daniela Vieira Buchaim, and João Paulo Mardegan Issa. 2025. "Vitamin D in Peri-Implant and Periodontal Tissue" Dentistry Journal 13, no. 10: 448. https://doi.org/10.3390/dj13100448
APA StyleDuarte, F. d. S., Duarte, N. D., Mulinari-Santos, G., Frigério, P. B., Okamoto, R., Buchaim, R. L., Buchaim, D. V., & Issa, J. P. M. (2025). Vitamin D in Peri-Implant and Periodontal Tissue. Dentistry Journal, 13(10), 448. https://doi.org/10.3390/dj13100448