Next Article in Journal
An Unknown Hidden Target Localization Method Based on Data Decoupling in Complex Scattering Media
Next Article in Special Issue
Enhancement of Imaging Quality of Interferenceless Coded Aperture Correlation Holography Based on Physics-Informed Deep Learning
Previous Article in Journal
Laser Driven Electron Acceleration from Near-Critical Density Targets towards the Generation of High Energy γ-Photons
Previous Article in Special Issue
Watermarking and Encryption for Holographic Communication
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Photonics
Volume 9
Issue 12
10.3390/photonics9120955
photonics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry

by
Marek Feith
1,2,†,
Yuecheng Zhang
2,3,4,
Jenny L. Persson
2,3,5,
Jan Balvan
1,
Zahra El-Schich
2,3 and
Anette Gjörloff Wingren
2,3,*
1
Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic
2
Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, Sweden
3
Biofilms Research Center for Biointerfaces, Malmö University, 205 06 Malmö, Sweden
4
College of Chemistry and Chemical Engineering, Yan’an University, Yan’an 215123, China
5
Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden
*
Author to whom correspondence should be addressed.
†
Current Address: Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital—The Norwegian Radium Hospital, 0424 Oslo, Norway.
Photonics 2022, 9(12), 955; https://doi.org/10.3390/photonics9120955
Submission received: 3 November 2022 / Revised: 5 December 2022 / Accepted: 7 December 2022 / Published: 9 December 2022
(This article belongs to the Special Issue Advances and Application of Imaging on Digital Holography)
Browse Figures
Review Reports Versions Notes

Abstract

Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers.
Keywords: cell morphology; CD44; circulating tumor cell; colorectal cancer; digital holographic cytometry; EpCAM cell morphology; CD44; circulating tumor cell; colorectal cancer; digital holographic cytometry; EpCAM
Graphical Abstract

Share and Cite

MDPI and ACS Style

Feith, M.; Zhang, Y.; Persson, J.L.; Balvan, J.; El-Schich, Z.; Wingren, A.G. Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry. Photonics 2022, 9, 955. https://doi.org/10.3390/photonics9120955

AMA Style

Feith M, Zhang Y, Persson JL, Balvan J, El-Schich Z, Wingren AG. Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry. Photonics. 2022; 9(12):955. https://doi.org/10.3390/photonics9120955

Chicago/Turabian Style

Feith, Marek, Yuecheng Zhang, Jenny L. Persson, Jan Balvan, Zahra El-Schich, and Anette Gjörloff Wingren. 2022. "Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry" Photonics 9, no. 12: 955. https://doi.org/10.3390/photonics9120955

APA Style

Feith, M., Zhang, Y., Persson, J. L., Balvan, J., El-Schich, Z., & Wingren, A. G. (2022). Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry. Photonics, 9(12), 955. https://doi.org/10.3390/photonics9120955

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop