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Open AccessLetter

A Robust Method for Adjustment of Laser Speckle Contrast Imaging during Transcranial Mouse Brain Visualization

1
Department of Veterinary Resources, Weizmann Institute of Science, 76100 Rehovot, Israel
2
Optoelectronics and Measurement Techniques Laboratory, University of Oulu, 90570 Oulu, Finland
3
Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, 634050 Tomsk, Russia
4
Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University (MEPhI), 115409 Moscow, Russia
5
Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham B4 7ET, UK
6
School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK
*
Author to whom correspondence should be addressed.
These two authors contributed equally to this work.
Photonics 2019, 6(3), 80; https://doi.org/10.3390/photonics6030080
Received: 11 June 2019 / Revised: 4 July 2019 / Accepted: 10 July 2019 / Published: 13 July 2019
(This article belongs to the Special Issue Biomedical Photonics Advances)
Laser speckle imaging (LSI) is a well-known and useful approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues (such as brain vasculature, skin capillaries etc.). Despite an extensive use of LSI for brain imaging, the LSI technique has several critical limitations. One of them is associated with inability to resolve a functionality of vessels. This limitation also leads to the systematic error in the quantitative interpretation of values of speckle contrast obtained for different vessel types, such as sagittal sinus, arteries, and veins. Here, utilizing a combined use of LSI and fluorescent intravital microscopy (FIM), we present a simple and robust method to overcome the limitations mentioned above for the LSI approach. The proposed technique provides more relevant, abundant, and valuable information regarding perfusion rate ration between different types of vessels that makes this method highly useful for in vivo brain surgical operations. View Full-Text
Keywords: cerebral blood vessels; middle cerebral artery; transcranial imaging; in vivo fluorescence imaging; non-invasive optical imaging; speckle contrast cerebral blood vessels; middle cerebral artery; transcranial imaging; in vivo fluorescence imaging; non-invasive optical imaging; speckle contrast
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Kalchenko, V.; Sdobnov, A.; Meglinski, I.; Kuznetsov, Y.; Molodij, G.; Harmelin, A. A Robust Method for Adjustment of Laser Speckle Contrast Imaging during Transcranial Mouse Brain Visualization. Photonics 2019, 6, 80.

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