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Open AccessArticle

Exploring the Potential of Airyscan Microscopy for Live Cell Imaging

1
Kennedy Institute for Rheumatology, Roosevelt Drive, University of Oxford, Oxford OX3 7LF, UK
2
Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK
3
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK
*
Author to whom correspondence should be addressed.
Photonics 2017, 4(3), 41; https://doi.org/10.3390/photonics4030041
Received: 1 June 2017 / Revised: 19 June 2017 / Accepted: 30 June 2017 / Published: 7 July 2017
(This article belongs to the Special Issue Superresolution Optical Microscopy)
Biological research increasingly demands the use of non-invasive and ultra-sensitive imaging techniques. The Airyscan technology was recently developed to bridge the gap between conventional confocal and super-resolution microscopy. This technique combines confocal imaging with a 0.2 Airy Unit pinhole, deconvolution and the pixel-reassignment principle in order to enhance both the spatial resolution and signal-to-noise-ratio without increasing the excitation power and acquisition time. Here, we present a detailed study evaluating the performance of Airyscan as compared to confocal microscopy by imaging a variety of reference samples and biological specimens with different acquisition and processing parameters. We found that the processed Airyscan images at default deconvolution settings have a spatial resolution similar to that of conventional confocal imaging with a pinhole setting of 0.2 Airy Units, but with a significantly improved signal-to-noise-ratio. Further gains in the spatial resolution could be achieved by the use of enhanced deconvolution filter settings, but at a steady loss in the signal-to-noise ratio, which at more extreme settings resulted in significant data loss and image distortion. View Full-Text
Keywords: Airyscan; microscopy; confocal and super-resolution microscopy; signal-to-noise-ratio Airyscan; microscopy; confocal and super-resolution microscopy; signal-to-noise-ratio
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MDPI and ACS Style

Korobchevskaya, K.; Lagerholm, B.C.; Colin-York, H.; Fritzsche, M. Exploring the Potential of Airyscan Microscopy for Live Cell Imaging. Photonics 2017, 4, 41. https://doi.org/10.3390/photonics4030041

AMA Style

Korobchevskaya K, Lagerholm BC, Colin-York H, Fritzsche M. Exploring the Potential of Airyscan Microscopy for Live Cell Imaging. Photonics. 2017; 4(3):41. https://doi.org/10.3390/photonics4030041

Chicago/Turabian Style

Korobchevskaya, Kseniya; Lagerholm, B. C.; Colin-York, Huw; Fritzsche, Marco. 2017. "Exploring the Potential of Airyscan Microscopy for Live Cell Imaging" Photonics 4, no. 3: 41. https://doi.org/10.3390/photonics4030041

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