Next Article in Journal
Development of Green and Efficient Extraction Methods of Quercetin from Red Onion Scales Wastes Using Factorial Design for Method Optimization: A Comparative Study
Next Article in Special Issue
Development of a Method for the Measurement of Human Scent Samples Using Comprehensive Two-Dimensional Gas Chromatography with Mass Detection
Previous Article in Journal
A Simple Microextraction Method for Toxic Industrial Dyes Using a Fatty-Acid Solvent Mixture
Previous Article in Special Issue
Analysis of Floral Fragrance Compounds of Chimonanthus praecox with Different Floral Colors in Yunnan, China
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Separations
Volume 8
Issue 9
10.3390/separations8090136
separations-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study

by
Dyah K. Agustika
1,2,*,
Ixora S. Mercuriani
3,
Nur Aeni Ariyanti
3,
Chandra W. Purnomo
4,
Kuwat Triyana
5,
Daciana D. Iliescu
1 and
Mark S. Leeson
1
1
School of Engineering, University of Warwick, Coventry CV4 7AL, UK
2
Department of Physics Education, Universitas Negeri Yogyakarta, Yogyakarta 55281, Indonesia
3
Department of Biology Education, Universitas Negeri Yogyakarta, Yogyakarta 55281, Indonesia
4
Department of Chemical Engineering, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
5
Department of Physics, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
*
Author to whom correspondence should be addressed.
Separations 2021, 8(9), 136; https://doi.org/10.3390/separations8090136
Submission received: 24 July 2021 / Revised: 23 August 2021 / Accepted: 25 August 2021 / Published: 27 August 2021
(This article belongs to the Special Issue Comprehensive Separations of Complex Mixtures by Gas Chromatography/Mass Spectrometry)
Browse Figures
Versions Notes

Abstract

Pepper yellow leaf curl virus (PYLCV) is a threat to chili plants and can significantly reduce yields. This study aimed as a pilot project to detect PYLCV by analyzing compounds emitted by chili plants using gas chromatography-mass spectrometry (GC-MS). The samples investigated in this research were PYLCV-infected and PYLCV-undetected chili plants taken from commercial chili fields. The infection status was validated by using a polymerase chain reaction (PCR) test. A headspace technique was used to extract the volatile organic compounds emitted by plants. The analysis of GC-MS results began with pre-processing, analyzing sample compound variability with a boxplot analysis, and sample classification by using a multivariate technique. Unsupervised multivariate technique principal component analysis (PCA) was performed to discover whether GC-MS could identify PYLCV-infected or not. The results showed that PYLCV-infected and PYLCV-undetected chili plants could be differentiated, with a total percent variance of the first three principal components reaching 91.32%, and successfully discriminated between PYLCV-infected and PYLCV-undetected chili plants. However, more comprehensive studies are needed to find the potential biomarkers of the infected plants.
Keywords: pepper yellow leaf curl virus; plant disease detection; gas chromatography-mass spectrometry; multivariate analysis technique pepper yellow leaf curl virus; plant disease detection; gas chromatography-mass spectrometry; multivariate analysis technique

Share and Cite

MDPI and ACS Style

Agustika, D.K.; Mercuriani, I.S.; Ariyanti, N.A.; Purnomo, C.W.; Triyana, K.; Iliescu, D.D.; Leeson, M.S. Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study. Separations 2021, 8, 136. https://doi.org/10.3390/separations8090136

AMA Style

Agustika DK, Mercuriani IS, Ariyanti NA, Purnomo CW, Triyana K, Iliescu DD, Leeson MS. Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study. Separations. 2021; 8(9):136. https://doi.org/10.3390/separations8090136

Chicago/Turabian Style

Agustika, Dyah K., Ixora S. Mercuriani, Nur Aeni Ariyanti, Chandra W. Purnomo, Kuwat Triyana, Daciana D. Iliescu, and Mark S. Leeson. 2021. "Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study" Separations 8, no. 9: 136. https://doi.org/10.3390/separations8090136

APA Style

Agustika, D. K., Mercuriani, I. S., Ariyanti, N. A., Purnomo, C. W., Triyana, K., Iliescu, D. D., & Leeson, M. S. (2021). Gas Chromatography-Mass Spectrometry Analysis of Compounds Emitted by Pepper Yellow Leaf Curl Virus-Infected Chili Plants: A Preliminary Study. Separations, 8(9), 136. https://doi.org/10.3390/separations8090136

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop