Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Apparatus and Conditions
2.3. Calibrator and Control Preparation
2.4. Sample Pretreatment and Extraction
2.5. Method Validation
2.5.1. Recovery, Matrix effect and Carry over
2.5.2. Linearity, Sensitivity and Selectivity
2.5.3. Stability
2.5.4. Accuracy and Repeatability
3. Results
3.1. Recovery, Matrix Effect, Carry Over
3.2. Linearity, Sensitivity and Selectivity
3.3. Stability
3.4. Accuracy and Repeatability
3.5. Application in real samples
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
- Schwartz, M.L.; Meyer, M.B.; Covino, B.G.; Narang, R.M.; Sethi, V.; Schwartz, A.J.; Kamp, P. Antiarrhythmic Effectiveness of Intramus-cular Lidocaine: Influence of Different Injection Sites. J. Clin. Pharmacol. 1974, 14, 77–83. [Google Scholar] [CrossRef] [PubMed]
- Paul, D.; Dan, C.; Schwartz, B.; Cooper, R.; Gelaznikas, R.; Barr, A. Amiodarone as Compared with Lidocaine for Shock-Resistant Ventricular Fibrillation. N. Engl. J. Med. 2002, 21, 884–890. [Google Scholar]
- Moriya, F.; Hashimoto, Y. Postmortem diffusion of tracheal lidocaine into heart blood following intubation for cardiopul-monary resuscitation. J. Forensic Sci. 1997, 42, 296–299. [Google Scholar] [CrossRef] [PubMed]
- Moriya, F.; Hashimoto, Y. Absorption of Intubation-Related Lidocaine from the Trachea during Prolonged Cardiopulmonary Resuscitation. J. Forensic Sci. 1998, 43, 718–722. [Google Scholar] [CrossRef] [PubMed]
- Moriya, F.; Hashimoto, Y. Determining the state of the deceased during cardiopulmonary resuscitation from tissue distri-bution patterns of intubation-related lidocaine. J. Forensic Sci. 2000, 45, 846–849. [Google Scholar] [CrossRef] [PubMed]
- Moriya, F.; Hashimoto, Y. Tissue distribution of lidocaine in critical care patients after intubation. Forensic Sci. Int. 2003, 137, 183–187. [Google Scholar] [CrossRef]
- Moriya, F.; Hashimoto, Y. Tissue Distribution of Intubation-Related Lidocaine in Brain-Dead Patients. Am. J. Forensic Med. Pathol. 2004, 25, 351–354. [Google Scholar] [CrossRef] [PubMed]
- Moriya, F.; Hashimoto, Y.; Nakanishi, A. Distributions of ethanol and intubation-related lidocaine in the brain of a trauma patient who was brain dead for about 5 days. Leg. Med. 2003, 5, S118–S122. [Google Scholar] [CrossRef]
- Moriya, F.; Hashimoto, Y. Concentrations of monoethylglycinexylidide in body fluids of deceased patients after use of li-docaine for endotracheal intubation. Leg. Med. 2000, 2, 31–35. [Google Scholar] [CrossRef]
- Benowitz, N.L.; Meister, W. Clinical pharmacokinetics of lignocaine. Clin. Pharmacokinet. 1978, 3, 177–201. [Google Scholar] [CrossRef] [PubMed]
- Wunder, C.; Meier, J.; Reyher, C.; Könitz, V.; Paulke, A.; Zacharowski, K.; Toennes, S.W. Use of lidocaine in endotracheal intubation. Blood and urine concentrations in patients and deceased after unsuccessful resuscitation. Forensic Sci. Int. 2014, 244, 259–262. [Google Scholar] [CrossRef] [PubMed]
- Barbaro, A. Teatino About an unusual case: GC-MS detection of lidocaine. Forensic Sci. Int. 2004, 146, S93–S94. [Google Scholar]
- Centini, F.; Fiore, C.; Riezzo, I.; Rossi, G.; Fineschi, V. Suicide due to oral ingestion of lidocaine: A case report and review of the literature. Forensic Sci. Int. 2007, 171, 57–62. [Google Scholar] [CrossRef] [PubMed]
- Zhang, N.; Sun, J.; Wei, Z.; He, W.; Jin, G.; Zhang, X.; Gao, P.; Wang, L.; Yun, K. Comparison of the Concentrations of Lido-caine in Different Body Fluids/Tissues after Subarachnoid Space and Intravenous Administration of a Lethal Dose of Lido-caine. J. Forensic. Sci. Med. 2015, 1, 48–53. [Google Scholar]
- Scientific Working Group for Forensic Toxicology (SWGTOX) Standard Practices for Method Validation in Forensic Toxicology. J. Anal. Toxicol. 2013, 37, 452–474. [CrossRef] [PubMed]
- Wei, Z.; Yu, J.; Zhang, C.; Jia, J.; Cao, J.; Wang, Y.; Gao, C.; Wang, Y.; Yun, K. Postmortem Redistribution of Lidocaine after Epidural Anesthesia Accidental Death. J. Forensic Res. 2017, 8, 2. [Google Scholar] [CrossRef]
- Zhang, C.; Liang, B.; Wang, C.; Wei, Z.; Yun, K. Postmortem redistribution of lidocaine after epidural injection in beagle dogs. Int. J. Clin. Exp. Med. 2015, 8, 18500–18505. [Google Scholar] [PubMed]
- Han, E.; Kim, E.; Hong, H.; Jeong, S.; Kim, J.; In, S.; Chung, H.; Lee, S. Evaluation of postmortem redistribution phenomena for commonly encountered drugs. Forensic Sci. Int. 2012, 219, 265–271. [Google Scholar] [CrossRef] [PubMed]
Validation Parameters | 0.25 μg/mL (LQC) | 1.25 μg/mL (MQC) | 5.00 μg/mL (HQC) |
---|---|---|---|
Recovery % | 106 | 112 | 103 |
Matrix Effect % | 9.7 | 12.6 | 8.3 |
Accuracy% | 101 | 105 | 99 |
Intraday repeatability (RSD%) | 7.1 | 10.4 | 8.3 |
Day-to-day repeatability (RSD%) | 5.9 | 2.3 | 4.2 |
Case | Male (M)/Female (F) | Age | Weight (Kg) | Cause of Death |
---|---|---|---|---|
1 | M | 17 | 70 | Hemorrhagic shock—traffic accident |
2 | M | 27 | 80 | Hemorrhagic shock—gunshot injury |
3 | M | 23 | 75 | Hemorrhagic shock—traffic accident |
4 | M | 42 | 78 | Respiratory infection—coronary atherosclerosis |
5 | M | 84 | 85 | Hypertrophic cardiomyopathy—pulmonary edema |
6 | F | 46 | 62 | Myocardial infarction—coronary atherosclerosis |
7 | M | 48 | 75 | Myocardial infarction—coronary atherosclerosis |
8 | M | 51 | 80 | Aortic dissection |
9 | F | 39 | 110 | Cardiac arrest—epilepsy |
10 | M | 59 | 70 | Pericarditis—hypertrophic cardiomyopathy |
11 | M | 38 | 80 | Myocardial infarction—coronary atherosclerosis |
12 | M | 46 | 75 | Respiratory infection—coronary atherosclerosis |
13 | F | 27 | 58 | Hemorrhagic shock—traffic accident |
14 | F | 49 | 60 | Myocardial infarction—coronary atherosclerosis |
15 | M | 65 | 110 | Hemorrhagic shock—gunshot injury |
16 | M | 65 | 72 | Myocardial infarction—right coronary artery thrombosis |
17 | M | 56 | 85 | Hypertensive heart disease |
18 | M | 68 | 80 | Drowning—ischemic heart disease |
19 | M | 41 | 84 | Myocardial infarction—coronary atherosclerosis |
20 | M | 80 | 78 | Ischemic heart disease—gastric cancer |
21 | F | 49 | 62 | Aortic dissection |
22 | M | 26 | 95 | Craniocerebral gunshot injury |
23 | M | 85 | 60 | Hypertrophic cardiomyopathy |
Case No | Lidocaine (μg/mL) | Case No | Lidocaine (μg/mL) |
---|---|---|---|
1 | 0.27 | 14 | 0.89 |
2 | 0.96 | 15 | 0.38 |
3 | 0.90 | 16 | 0.25 |
4 | 0.21 | 17 | 0.36 |
5 | 0.36 | 18 | 0.27 |
6 | 0.32 | 19 | 0.22 |
7 | 0.29 | 20 | 0.32 |
8 | 0.58 | 21 | 0.37 |
9 | 0.88 | 22 | 0.27 |
10 | 0.72 | 23 | 0.25 |
11 | 0.30 | ||
12 | 0.40 | ||
13 | 0.23 |
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Orfanidis, A.; Raikos, N.; Brousa, E.; Zangelidou, E.; Mastrogianni, O. Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation. Separations 2021, 8, 117. https://doi.org/10.3390/separations8080117
Orfanidis A, Raikos N, Brousa E, Zangelidou E, Mastrogianni O. Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation. Separations. 2021; 8(8):117. https://doi.org/10.3390/separations8080117
Chicago/Turabian StyleOrfanidis, Amvrosios, Nikolaos Raikos, Evdokia Brousa, Eleni Zangelidou, and Orthodoxia Mastrogianni. 2021. "Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation" Separations 8, no. 8: 117. https://doi.org/10.3390/separations8080117