Next Article in Journal
Simultaneous Determination of C18 Fatty Acids in Milk by GC-MS
Previous Article in Journal
Evaluation of High Resolution Melting (HRM) Analysis for Meat Species Identification of Raw and Cooked Meat
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Separations
Volume 8
Issue 8
10.3390/separations8080117
Review Report
separations-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation

Separations 2021, 8(8), 117; https://doi.org/10.3390/separations8080117
by Amvrosios Orfanidis 1, Nikolaos Raikos 1, Evdokia Brousa 1, Eleni Zangelidou 2 and Orthodoxia Mastrogianni 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Reviewer 3: Anonymous
Separations 2021, 8(8), 117; https://doi.org/10.3390/separations8080117
Submission received: 9 July 2021 / Revised: 30 July 2021 / Accepted: 7 August 2021 / Published: 9 August 2021
(This article belongs to the Section Forensics/Toxins)

Round 1

Reviewer 1 Report

General comments:

In this manuscript, the authors have developed GC-MS method for determination of lidocaine in postmortem whole blood using Liquid-Liquid Extraction for sample pre-treatment. The method was applied to real whole blood samples from cases where unsuccessful Cardiopulmonary Resuscitation was attempted.

My remarks and recommendations are as follows:

  1. The introduction should be described in more detail. More references to the existing methods for the determination of lidocaine in postmortem blood samples should be also provided. 
  2. Negligible novelty and significance of the research. There is a number of published analytical methods (also GC-MS) for determination of lidocaine in postmortem samples including blood. Nevertheless, the authors did not provide the comparison of the proposed method with already known ones. What is the novelty and advantages of this work compared to papers published earlier? Novelty and significance of the work should be explained and highlighted.
  3. It would be good to give some results of the statistical analysis regarding the results of quantification in real samples (e.g. mean, median, standard deviation).

Additionally:

  1. I don't think the abbreviation of Cardiopulmonary Resuscitation should be used in the title. In my opinion, the full name should be used.
  2. There are some typos in the text. For example: in abstract it should be written ,,Cardiopulmonary Resuscitation’’ not ,,CardiPulmonary Resuscitation’’. The text of manuscript should be carefully checked.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

from paper it is not clear what kind of guidelines were used to validate the method

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 3 Report

The article describes a method for the detection of lidocaine in postmortem whole blood using LLE followed by GC/MS analysis.

Lidocaine chemical structure should be presented. Pharmacological and pharmacokinetic data about lidocaine are missing in the article. The Introduction section is supefically written. What about other methods of detecting lidocaine in biological matrices ?

Statistical presentation of the results can be improved. Validation data should be included in a table for a more rapid identication by the reader.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

I accept the changes made to the manuscript and the authors' explanations of my earlier comments. Accordingly, I recommend manuscript for publication in the present form 

Reviewer 3 Report

The article can be published in the current form

Back to TopTop