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Review

Recent Advances in Chiral Analysis of Proteins and Peptides

by 1,2,* and 1,2,*
1
Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic
2
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editor: Maria Elizabeth Tiritan
Separations 2021, 8(8), 112; https://doi.org/10.3390/separations8080112
Received: 8 June 2021 / Revised: 27 July 2021 / Accepted: 27 July 2021 / Published: 29 July 2021
(This article belongs to the Special Issue Separations and Analysis of Proteins in Biological Samples)
Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain d-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with d-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer’s disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of d-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations. View Full-Text
Keywords: chiral separation; proteins; peptides; d-amino acids chiral separation; proteins; peptides; d-amino acids
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MDPI and ACS Style

Morvan, M.; Mikšík, I. Recent Advances in Chiral Analysis of Proteins and Peptides. Separations 2021, 8, 112. https://doi.org/10.3390/separations8080112

AMA Style

Morvan M, Mikšík I. Recent Advances in Chiral Analysis of Proteins and Peptides. Separations. 2021; 8(8):112. https://doi.org/10.3390/separations8080112

Chicago/Turabian Style

Morvan, Marine, and Ivan Mikšík. 2021. "Recent Advances in Chiral Analysis of Proteins and Peptides" Separations 8, no. 8: 112. https://doi.org/10.3390/separations8080112

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