Next Article in Journal
The Use of Ferrofluids in Analytical Sample Preparation: A Review
Next Article in Special Issue
Comparison of Adsorbents Containing Carbon Nanotubes for Express Pre-Concentration of Volatile Organic Compounds from the Air Flow
Previous Article in Journal
Cost-Effective Simultaneous Separation and Quantification of Phenolics in Green and Processed Tea Using HPLC–UV–ESI Single-Quadrupole MS Detector and Python Script
Previous Article in Special Issue
Rapid Determination of Ethylene Oxide and 75 VOCs in Ambient Air with Canister Sampling and Associated Growth Issues
Article

Eco-Friendly Direct GC–MS Method for Estimation of Niacin and Related Impurities Involving Pyridine in Food Supplements

1
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
2
Addiction and Neuroscience Research Unit, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
3
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., Beni-Suef 62514, Egypt
*
Author to whom correspondence should be addressed.
Academic Editor: Nicholas Snow
Separations 2021, 8(4), 46; https://doi.org/10.3390/separations8040046
Received: 14 February 2021 / Revised: 9 March 2021 / Accepted: 24 March 2021 / Published: 6 April 2021
(This article belongs to the Special Issue Rapid Separations of Complex Mixtures)
Niacin is a water-soluble vitamin whose deficiency causes many disorders and diseases, including pellagra and high blood cholesterol. Herein, niacin and four common impurities, isonicotinic acid (ISO), 5-ethyl-2-methylpyridine (MP), pyridine-2, 5-dicarboxylic acid (PDC) and pyridine PYR, are simultaneously analyzed, where PYR is known as potentially nephrotoxic and hepatotoxic. The separation of a mixture using gas chromatography–mass spectroscopy (GC–MS) without any derivatization steps was the main target. Many studies have been published to study Niacin and its impurities using colorimetry and HPLC. GC–MS was selected to study the analyzed mixture owing to its known sensitivity and selectivity. In this study, a single quadrupole mass spectrometer operated in selected ion monitoring (SIM) mode at an electron ionization energy of 70 eV was applied for the quantitative analysis of Niacin. Helium was used as the carrier gas and adjusted to run through an HP-5ms (5%-phenyl)-methylpolysiloxane column. Statistical analysis proved that this method is equally effective as the previously reported method. Importantly, this study was eco-friendly as compared to the reported high performance thin layer chromatographic method (HPTLC). Finally, this study provides a new and valid eco-friendly method analysis to determine the concentration of niacin and its common impurities at very low concentration. Conclusion: A simple, rapid accurate and green GC–MS analytical method was developed and validated to determine niacin and its related official impurities. View Full-Text
Keywords: gas chromatography–mass spectroscopy; niacin; pyridine; selected ion monitoring gas chromatography–mass spectroscopy; niacin; pyridine; selected ion monitoring
Show Figures

Figure 1

MDPI and ACS Style

Almalki, A.H.; Naguib, I.A.; Abdallah, F.F. Eco-Friendly Direct GC–MS Method for Estimation of Niacin and Related Impurities Involving Pyridine in Food Supplements. Separations 2021, 8, 46. https://doi.org/10.3390/separations8040046

AMA Style

Almalki AH, Naguib IA, Abdallah FF. Eco-Friendly Direct GC–MS Method for Estimation of Niacin and Related Impurities Involving Pyridine in Food Supplements. Separations. 2021; 8(4):46. https://doi.org/10.3390/separations8040046

Chicago/Turabian Style

Almalki, Atiah H., Ibrahim A. Naguib, and Fatma F. Abdallah. 2021. "Eco-Friendly Direct GC–MS Method for Estimation of Niacin and Related Impurities Involving Pyridine in Food Supplements" Separations 8, no. 4: 46. https://doi.org/10.3390/separations8040046

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop