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10.3390/separations8120238
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Article

A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay

by
Xiu Wang
1,2,
Yupei Zhang
1,2,
Nan Wu
1,2,
Jingya Cao
1,2,
Yanduo Tao
1 and
Ruitao Yu
1,*
1
Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Separations 2021, 8(12), 238; https://doi.org/10.3390/separations8120238
Submission received: 17 September 2021 / Revised: 16 November 2021 / Accepted: 30 November 2021 / Published: 6 December 2021
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Abstract

Free radicals, including 1,1-diphenyl-2-picrylhydrazyl, mediate oxidative stress to cause many chronic diseases (including cardiovascular diseases, diabetes and cancer). The extract of traditional Tibetan medicine Lepidium latifolium L. (L. latifolium) was reported to have free radical inhibition ability. Therefore, a system method was established to separate the ethanol extract of L. latifolium to prepare two main antioxidant compounds. First of all, silica gel and a medium-pressure liquid chromatography tower were used for pre-treatment of the ethanol extract of L. latifolium to obtain the main antioxidant active component fraction 4 through online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH) assay. Then, fraction 4-1 was obtained by one-dimensional preparation using Megres C18 chromatographic column, and two active compounds with IC50 values 59.9 and 71.3 μg/mL were obtained by two-dimensional preparation using Click XIon chromatographic column. Through the study of the chemical components and separation methods of L. latifolium, the combination of HPLC-DPPH assay and two-dimensional preparative liquid chromatography was realized, providing a reference for the separation of active compounds from L. latifolium.
Keywords: antioxidant activity; Lepidium latifolium L.; medium-pressure liquid chromatography; online HPLC-DPPH assay antioxidant activity; Lepidium latifolium L.; medium-pressure liquid chromatography; online HPLC-DPPH assay
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MDPI and ACS Style

Wang, X.; Zhang, Y.; Wu, N.; Cao, J.; Tao, Y.; Yu, R. A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay. Separations 2021, 8, 238. https://doi.org/10.3390/separations8120238

AMA Style

Wang X, Zhang Y, Wu N, Cao J, Tao Y, Yu R. A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay. Separations. 2021; 8(12):238. https://doi.org/10.3390/separations8120238

Chicago/Turabian Style

Wang, Xiu, Yupei Zhang, Nan Wu, Jingya Cao, Yanduo Tao, and Ruitao Yu. 2021. "A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay" Separations 8, no. 12: 238. https://doi.org/10.3390/separations8120238

APA Style

Wang, X., Zhang, Y., Wu, N., Cao, J., Tao, Y., & Yu, R. (2021). A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay. Separations, 8(12), 238. https://doi.org/10.3390/separations8120238

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