HILIC-MS/MS Analysis of Adenosine in Patient Blood

Virgiliou, Christina; Fragakis, Nikolaos; Sotiriadou, Melani; Vassilikos, Vassilios; Gerou, Spiros; Theodoridis, Georgios; Gika, Helen

doi:10.3390/separations8110222

Open AccessArticle

HILIC-MS/MS Analysis of Adenosine in Patient Blood

by

Christina Virgiliou

^1,2,*

,

Nikolaos Fragakis

³,

Melani Sotiriadou

³,

Vassilios Vassilikos

³

,

Spiros Gerou

⁴,

Georgios Theodoridis

^1,2

and

Helen Gika

^1,5

¹

Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece

²

BIOMIC_Auth, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Β1.4, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, 57001 Thessaloniki, Greece

³

3rd Cardiology Department, Hippokration General Hospital, Aristotle University Medical School, Konstantinoupoleos 49, 546 42 Thessaloniki, Greece

⁴

Analysis Laboratories, 54124 Thessaloniki, Greece

⁵

Laboratory of Forensic Medicine and Toxicology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece

^*

Author to whom correspondence should be addressed.

Separations 2021, 8(11), 222; https://doi.org/10.3390/separations8110222

Submission received: 4 October 2021 / Revised: 7 November 2021 / Accepted: 15 November 2021 / Published: 17 November 2021

(This article belongs to the Special Issue Application of Separations Technologies in Biomedical Analysis)

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Abstract

Adenosine is a purine ribonucleoside with important roles in various physiological processes. A number of studies have indicated the importance of adenosine in cardiovascular diseases including syncope; however, the accurate determination of adenosine in human blood is challenging due to the molecule’s instability. In the present study, we report a simple method for the pre-treatment of blood samples and the development of a fast and efficient hydrophilic interaction chromatographic tandem mass spectrometry method for the analysis of adenosine in patient blood. During collection, samples were mixed directly with a solvent mixture containing 95% acetonitrile and 10 mM ammonium formate in a Vacutainer tube, resulting in successful prevention of adenosine metabolic processes and direct blood sample deproteinization. The method was validated according to bioanalytical industry guidelines and found to be accurate, repeatable, specific and sensitive with LLOQ 0.005 μg/mL, thus allowing its application in the analysis of real clinical samples.

Keywords: adenosine; HILIC-MS/MS; blood; bioanalysis; syncope

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MDPI and ACS Style

Virgiliou, C.; Fragakis, N.; Sotiriadou, M.; Vassilikos, V.; Gerou, S.; Theodoridis, G.; Gika, H. HILIC-MS/MS Analysis of Adenosine in Patient Blood. Separations 2021, 8, 222. https://doi.org/10.3390/separations8110222

AMA Style

Virgiliou C, Fragakis N, Sotiriadou M, Vassilikos V, Gerou S, Theodoridis G, Gika H. HILIC-MS/MS Analysis of Adenosine in Patient Blood. Separations. 2021; 8(11):222. https://doi.org/10.3390/separations8110222

Chicago/Turabian Style

Virgiliou, Christina, Nikolaos Fragakis, Melani Sotiriadou, Vassilios Vassilikos, Spiros Gerou, Georgios Theodoridis, and Helen Gika. 2021. "HILIC-MS/MS Analysis of Adenosine in Patient Blood" Separations 8, no. 11: 222. https://doi.org/10.3390/separations8110222

APA Style

Virgiliou, C., Fragakis, N., Sotiriadou, M., Vassilikos, V., Gerou, S., Theodoridis, G., & Gika, H. (2021). HILIC-MS/MS Analysis of Adenosine in Patient Blood. Separations, 8(11), 222. https://doi.org/10.3390/separations8110222

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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HILIC-MS/MS Analysis of Adenosine in Patient Blood

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