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Open AccessFeature PaperArticle

Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

1
Department for biomedical and inorganic chemistry, LNE, 75015 Paris, France
2
Experimental cancerology laboratory, CEA, 92260 Fontenay-aux-Roses, France
3
Laboratoire de Chimie Physique, UMR CNRS 8000, Université Paris Sud, 91405 Orsay, France
4
National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
*
Author to whom correspondence should be addressed.
Separations 2019, 6(1), 3; https://doi.org/10.3390/separations6010003
Received: 31 October 2018 / Revised: 22 November 2018 / Accepted: 13 December 2018 / Published: 9 January 2019
(This article belongs to the Special Issue Analytic Techniques for Nano- and Microparticles Analysis)
Gold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and to understand their mechanisms of action. However, using nanoparticles in such studies requires thorough knowledge of their cellular uptake. In this study, we optimized single particle ICPMS (sp-ICPMS) analysis to qualify and quantify intracellular AuNP content after exposure of in vitro human breast cancer cell lines. To this aim, cells were treated with an alkaline digestion method with 5% TMAH, allowing the detection of gold with a yield of 97% on average. Results showed that under our experimental conditions, the AuNP size distribution appeared to be unchanged after internalization and that the uptake of particles depended on the cell line and on the exposure duration. Finally, the comparison of the particle numbers per cell with the estimates based on the gold masses showed excellent agreement, confirming the validity of the sp-ICPMS particle measurements in such complex samples. View Full-Text
Keywords: sp-ICPMS; gold nanoparticles; tumor cells; nanoparticle uptake sp-ICPMS; gold nanoparticles; tumor cells; nanoparticle uptake
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Noireaux, J.; Grall, R.; Hullo, M.; Chevillard, S.; Oster, C.; Brun, E.; Sicard-Roselli, C.; Loeschner, K.; Fisicaro, P. Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS. Separations 2019, 6, 3.

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