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Open AccessArticle

Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids

Czech Academy of Sciences, Institute of Analytical Chemistry, Veveri 97, Brno 60200, Czech Republic
Author to whom correspondence should be addressed.
Separations 2018, 5(4), 48;
Received: 27 June 2018 / Revised: 31 August 2018 / Accepted: 5 September 2018 / Published: 2 October 2018
(This article belongs to the Special Issue Advances in Capillary Liquid Chromatography)
A strategy for the preparation of silica-based monolithic capillary columns (150 × 0.1 mm) with high selectivity to amino acids is presented. The zwitterionic columns were prepared by coating the silica monolith with [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. The columns were evaluated under isocratic conditions in hydrophilic interaction liquid chromatography. The best separation of amino acids was obtained on the monolithic column prepared by a stepwise modification procedure where the modification step was repeated four times. The mixture of fifteen amino acids was separated within 13 min using the mobile phase consisting of 75% acetonitrile and 25% 5 mmol/L ammonium acetate at pH 4.5. View Full-Text
Keywords: HILIC; amino acids; silica monolithic column HILIC; amino acids; silica monolithic column
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Moravcová, D.; Planeta, J. Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids. Separations 2018, 5, 48.

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