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Open AccessArticle

Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids

Czech Academy of Sciences, Institute of Analytical Chemistry, Veveri 97, Brno 60200, Czech Republic
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Separations 2018, 5(4), 48; https://doi.org/10.3390/separations5040048
Received: 27 June 2018 / Revised: 31 August 2018 / Accepted: 5 September 2018 / Published: 2 October 2018
(This article belongs to the Special Issue Advances in Capillary Liquid Chromatography)
A strategy for the preparation of silica-based monolithic capillary columns (150 × 0.1 mm) with high selectivity to amino acids is presented. The zwitterionic columns were prepared by coating the silica monolith with [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. The columns were evaluated under isocratic conditions in hydrophilic interaction liquid chromatography. The best separation of amino acids was obtained on the monolithic column prepared by a stepwise modification procedure where the modification step was repeated four times. The mixture of fifteen amino acids was separated within 13 min using the mobile phase consisting of 75% acetonitrile and 25% 5 mmol/L ammonium acetate at pH 4.5. View Full-Text
Keywords: HILIC; amino acids; silica monolithic column HILIC; amino acids; silica monolithic column
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Moravcová, D.; Planeta, J. Monolithic Silica Capillary Columns with Improved Retention and Selectivity for Amino Acids. Separations 2018, 5, 48.

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