DNA-immobilized silica gel TLC plate (DNA-TLC plate) by coating a mixture of double-stranded DNA and silica gel on a glass plate was stable in common organic solvents, such as alcohol, acetone, chloroform, and ethyl acetate. DNA-TLC plate showed different Rf
values for the L- and D-form acidic amino acids, such as aspartic acid and glutamic acid. The chiral recognition of an acidic amino acid by the DNA-TLC was related to the interaction between the nucleic acid base, particularly guanine (G) and cytosine (C), and the amino acid. Furthermore, the DNA-TLC plate indicated the recognition of a chiral metal complex, such as the tris(1,10-phenanthroline)ruthenium(II) ([Ru(phen)3
) salt, which has Λ- and Δ-forms. Therefore, the double-stranded DNA-TLC plate may have the potential to be utilized as a chiral separation material for amino acids, peptides, and a metal complex with a chiral property.
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